| 引用本文: | 李国成,刘春霞,余晓霞.HPLC测定祛痰合剂中橙皮苷的含量[J].中国现代应用药学,2011,28(13):1353-1355. |
| LI Guocheng,LIU Chunxia,YU Xiaoxia.Determination of Hesperdin in Expectorant Mixture by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(13):1353-1355. |
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| 摘要: |
| 目的 建立高效液相色谱法测定祛痰合剂中橙皮苷的含量。方法 采用Agilent Eclipse XDB-C18柱(250 mm×4.6 mm，5 μm)，流动相为甲醇-冰醋酸-水(25∶5∶70)，流速1 mL·min-1，检测波长283 nm，柱温30 ℃。结果 橙皮苷线性范围是1.05~34.56 μg·mL-1，线性方程为Y=10.346X+0.795 2，r=0.999 6；平均回收率为98.8%，RSD为1.2%(n=9) 。结论 本法简便，准确和选择性好，可用于祛痰合剂的质量控制。 |
| 关键词: 高效液相色谱法 祛痰合剂 橙皮苷 |
| DOI： |
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| Determination of Hesperdin in Expectorant Mixture by HPLC |
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LI Guocheng, LIU Chunxia, YU Xiaoxia
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Department of Pharmacy, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
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| Abstract: |
| OBJECTIVE An HPLC method was developed and validated for determining hesperdin in Expectorant mixture. METHODS Chromatographic separation was performed on an Agilent Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm) with a mobile phase of methanol-glacial acetic acid-distilled water(25∶5∶70). The flow rate was 1.0 mL·min-1 and the detecting wavelength was 283 nm. RESULTS The linear range of hesperdin was 1.05-34.56 μg·mL-1. Linear equation was Y=10.346X+0.795 2(r=0.999 6). The average recovery (n=9) of hesperdin was 98.3% (RSD=1.7%). CONCLUSION The method is simple, accurate and selective and sensitive to quantify hesperdin in Expectorant mixture. |
| Key words: HPLC Expectorant mixture hesperdin |
