虫草多糖对OVA过敏性哮喘鼠气道炎症和气道高反应的影响

Effects of Cordyceps Polysaccharide on the OVA-induced Airway Inflammation and Hyperresponsiveness in a Murine Asthmatic Model

关键词:  虫草多糖  哮喘  气道炎症  气道高反应

Key words:  Cordyceps polysaccharide  asthma  airway inflammation  airway hyperresponsiveness

摘要:

目的 观察虫草多糖是否具有抗过敏性哮喘的作用。方法 建立小鼠哮喘模型，第0，14天，分别腹腔注射OVA及其佐剂，自21 d时开始雾化吸入OVA一次，连续14 d，吸入OVA前0.5 h，观察组予虫草多糖100 mg·kg^-1灌胃，阳性药物腹腔注射地塞米松。最后一次OVA雾化吸入后12 h进行气道高反应实验，之后收集支气管灌洗液进行细胞计数、分类、ELISA和RT-PCR检测，取左下肺组织做病理及免疫组化，观察肺组织炎症改变。结果 ①哮喘组气道反应性较正常组显著增高，虫草多糖治疗组气道反应性显著降低(P<0.05)。②病理组织分析哮喘组支气管和小血管周围显示明显的嗜酸性粒细胞浸润，支气管黏液分泌增加；虫草多糖治疗组支气管和小血管嗜酸性粒细胞浸润程度明显减轻，支气管黏液分泌减少。③哮喘组BALF中出现大量嗜酸性粒细胞，虫草多糖治疗组较哮喘组嗜酸性粒细胞明显减少(P<0.05)。④哮喘组支气管灌洗液中IL-4、IL-5、IL-13较正常组显著增加，虫草多糖治疗组较哮喘组明显降低(P<0.05)。⑤哮喘组肺组织中IL-4、IL-5、IL-13 mRNA表达增加，虫草多糖治疗组较哮喘组表达降低。结论 虫草多糖治疗可在一定程度上减轻哮喘小鼠的气道嗜酸性粒细胞炎症及粘痰蛋白的分泌。

Abstract:

OBJECTIVE To observe the effects of Cordyceps polysaccharide on the antigen-induced airway eosinophilic inflammation. METHODS Thirty-two female BALB/c mice were randomly divided into four groups: control, asthma model, Cordyceps polysaccharide and Dexamethasone treatment groups. Asthma was induced by OVA administration in the asthma model, Cordyceps polysaccharide and Dexamethasone treatment groups. Cordyceps polysaccharide and Dexamethasone were administered before antigen challenge. After 12 hours of the last challenge, the mice were used to evaluate airway hyperresponsiveness(AHR). After the airway hyperresponsiveness experimentation , mice were killed and Bronchial alveolar lavage fluid (BALF) was collected to count the number of eosinophil and to measure the levels of IL-4, IL-5, IL-13. The lung tissues of inferior lobe of the left lung were obtained for the observation of lung pathological change. RESULTS ① Cordyceps polysaccharide significantly suppressed airway hyperresponsiveness in OVA-induced mice. ② Cordyceps polysaccharide attenuated the airway inflammation in the lung of OVA-induced mice. ③ In Cordyceps polysaccharide treatment groups, increase of eosinophil counts challenged by OVA was decreased in BALF of mice. ④ The increased levels of IL-4, IL-5, IL-13 challenged by OVA in BALF of mice were decreased by Cordyceps polysaccharide treatment. ⑤ The increased expressions of IL-4, IL-5, IL-13 mRNA challenged by OVA in lung tissues were also decreased by cordyceps polysaccharide treatment. CONCLUSION Cordyceps polysaccharide efficiently inhibited the eosinophil inflammation in lung tissue and may be of therapeutic value as a novel treatment of asthma.