HPLC测定降脂宁调脂抗氧化有效部位中槲皮素含量

Quantitative Determination of Quercetin in the Lipid-regulation and anti-Oxidation Effective Fraction of Jiangzhining by HPLC

关键词:  降脂宁  槲皮素  含量测定  高效液相色谱法

Key words:  Jiangzhining  quercetin  quantitative determination  HPLC

摘要:

目的 建立降脂宁调脂抗氧化有效部位中槲皮素的HPLC含量测定方法。方法 采用SunFire C₁₈色谱柱(150 mm × 4.6 mm，5 μm)，流动相为乙腈-0.1%磷酸水溶液(30︰70)，流速1.0 mL·min^-1，检测波长370 nm，柱温30 ℃。结果 槲皮素在0.052 2~0.313 2 μg内线性关系良好，回归方程为Y=3 231 057.74X－4 753.64(r=0.999 5)，平均回收率为99.36%，RSD=1.73%(n=6)。 结论 3批样品测定结果表明，该方法简便、准确，可用于降脂宁有效部位中槲皮素的含量测定。

Abstract:

OBJECTIVE To establish a method for the quantitative determination of quercetin in the lipid-regulation and anti-oxidation effective fraction of Jiangzhining. METHODS The determination was carried out with SunFire C₁₈ column (150 mm × 4.6 mm, 5 μm), using acetonitrile-0.1% phosphoric acid water solution (30︰70) as mobile phase at a flow rate of 1.0 mL·min^-1 and detected at the wavelength 370 nm, column temperature 30℃. RESULTS Quercetin had a good linear relationship in the range of 0.052 2-0.313 2 μg with the linear regression equation was Y=3 231 057.74X－4 753.64(r=0.999 5), the average recovery was 99.36% with RSD=1.73%(n=6). CONCLUSION The determination results of three batches of samples showed that the method was easy and accurate which could be used to determine the content of quercetin in the effective fraction of Jiangzhining.