| 引用本文: | 成英，宋九华.HPLC测定逍遥丸中甘草苷、异甘草素和甘草酸的含量[J].中国现代应用药学,2012,29(2):163-166. |
| CHENG Ying, SONG Jiuhua.Determination of Liquiritin, Glycyrrhizic Acid and Isoliquiritigenin in Xiaoyao Wan by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(2):163-166. |
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| 摘要: |
| 目的 建立测定逍遥丸中甘草苷、异甘草素和甘草酸含量的方法。方法 色谱柱：Shim-packVP-ODS(150 mm× 4.6 mm，5.0 μm)。流动相：甲醇-0.5%冰醋酸梯度洗脱；流速1 mL·min-1；检测波长0～7 min为276 nm，7～18 min为370 nm，18 min以后为254 nm。结果 甘草苷的线性范围是0.018 40～0.368 0 μg(r=0.999 8，平均回收率为97.96%，RSD为0.60%)；异甘草素的线性范围是0.015 2～0.304 0 μg(r=0.999 9，平均回收率为97.17%，RSD为1.02%)；甘草酸的线性范围是0.441 8～8.835 0 μg (r=0.999 9，平均回收率为97.52%，RSD为1.06%)。结论 所建立的方法简便可行、重复性好，可用于逍遥丸的质量控制。 |
| 关键词: 高效液相色谱法 逍遥丸 甘草苷 异甘草素 甘草酸 |
| DOI： |
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| 基金项目:四川省教育厅支持项目(10zB024) |
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| Determination of Liquiritin, Glycyrrhizic Acid and Isoliquiritigenin in Xiaoyao Wan by HPLC |
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CHENG Ying, SONG Jiuhua
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Center for Functional Molecular Design, College of Chemistry and Life Science, Leshan Normal University, Leshan 614004, China
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| Abstract: |
| OBJECTIVE To develop an HPLC method for the liquiritin, glycyrrhizic acid and isoliquiritigenin in Xiaoyao Wan. METHODS The column Shim-pack VP-ODS(150 mm×4.6 mm，5.0 μm)was used. The mobile phase consisted of methanol-0.5% acetic acid with a gradient elution, and the flow rate was 1.0 mL·min-1. The detection wavelengthes were 276 nm in 0-7 min, 370 nm in 7-18 min, 254 nm after 18 min．RESULTS The linear range of liquiritin was 0.018 40-0.368 0 μg, r=0.999 8. The average recovery was 97.96%, RSD=0.60%. The linear range of glycyrrhizic acid was 0.015 20-0.304 0 μg , r=0.999 9. The average recovery was 97.17%, RSD=1.02%. The linear range of isoliquiritigenin was 0.441 8-8.835 0 μg , r=0.999 9. The average recovery was 97.52%, RSD=1.06%. CONCLUSION This method is simple, feasible, and the repeatability is good. It can be used for quality control of Xiaoyao Wan. |
| Key words: HPLC Xiaoyao Wan liquiritin glycyrrhizic acid isoliquiritigenin |
