近红外光谱法快速测定山橿药材中球松素含量

Determination of Pinostrobin in Lindera Reflexa Helms by Near-infrared Spectroscopy Technique

关键词:  近红外光谱技术  山橿  球松素  定量分析模型

Key words:  near-infrared (NIR) diffuse reflectance spectroscopy  Lindera reflexa Hemsl  pinostrobin  quantitative analysis model

摘要:

目的 利用近红外光谱(NIR)技术建立一种山橿中球松素含量的快速分析方法。方法 以HPLC分析值为参照，采用近红外漫反射光谱技术采集66份山橿样品的近红外漫反射光谱，结合偏最小二乘法(PLS)建立了球松素含量的定量分析模型。结果 所建模型的相关系数(R²)、外部验证均方差(RMSEP)和内部交叉验证均方差(RMSECV)分别为0.992 31、0.076 5和0.450 0；验证集样品的NIR测得值与药典法测得值进行配对T检验，差异无统计学意义。结论 本方法操作简便、无污染、结果准确可靠，可用于山橿中球松素含量的快速测定。

Abstract:

OBJECTIVE To establish a rapid analytical method for pinostrobin in Lindera reflexa Helms by near-infrared(NIR) diffuse reflectance technique. METHODS Collecting NIR spectra by NIR diffuse reflectance spectroscopy with HPLC values anlysis as a reference to build calibration models by the partial least square(PLS). RESULTS The correlation and coefficients of the PLS calibration models of NIR spectroscopy for phillyrin was 0.992 31, the root-mean-square error of prediction(RMSEP) was 0.076 5, the root-mean-square error of cross-validation (RMSECV) was 0.450 0; There was no statistical difference between NIR prediction of validation samples and HPLC measurements tested by paried sample T test. CONCLUSION The method is rapid, nondestructive and accurate, and can be applied to the fast determination of pinostrobin in extractum forsythiae siccus.