HPLC测定不同批次、不同产地翼首草中齐墩果酸和熊果酸的含量

Determination of Oleanolic Acid and Orsolic Acid in Pterocephalus Hookeri from Different Batches and Different Regions by HPLC

关键词:  高效液相色谱法  翼首草  齐墩果酸  熊果酸  不同产地  含量测定

Key words:  HPLC  Pterocephalus hookeri  oleanolic acid  orsolic acid  different regions  content determination

摘要:

目的 测定不同批次、不同产地翼首草中齐墩果酸和熊果酸的含量。方法 色谱柱：Boston Symmetrix C₁₈柱(250 mm×4.6 mm，5 μm)，甲醇-0.1 mol·L^-1乙酸铵溶液(85∶15)，流速0.8 mL·min^-1，检测波长210 nm，柱温30 ℃。结果 齐墩果酸和熊果酸的线性范围分别为1.728~25.92 μg(r=0.999 9)、2.188~32.82 μg(r=0.999 9)，齐墩果酸的平均回收率为99.9%，RSD为2.12%，熊果酸的平均回收率为99.9%，RSD为1.66%。通过比较不同产地和批次翼首草样品的测定结果，可知不同产地和批次中翼首草中齐墩果酸和熊果酸的含量有着较大差别，其中甘肃、青海和四川西宁所产齐墩果酸和熊果酸含量远远高于其它产地。结论 该方法简便、准确、重复性好，试验结果为翼首草的药用研究、产品的开发提供了依据。

Abstract:

OBJECTIVE Determination of oleanolic acid and orsolic acid in Pterocephalus hookeri from different batches and different origins. METHODS The separation was performed on an ODS column with the mobile phase of CH₃OH-0.1 mol·L^-1 ammonium acetate solution. The flow rate was 0.8 mL·min^-1. The detection wavelength was 210 nm and the column temperature was 30 ℃. RESULTS The oleanolic acid and orsolic acid linearity range respectively was 1.728-25.92 μg(r=0.999 9), 2.188-32.82 μg(r=0.999 9), the average recovery of oleanolic acid was 99.9%, RSD was 2.12%, the ursolic acid average recovery was 99.9%, RSD was 1.66%. From the results obtained in this study, contents of oleanolic acid and orsolic acid differed from Pterocephalus hookeri collected from different batches and regions. And the contents of oleanolic acid and orsolic acid in Pterocephalus hookeri from Gansu, Qinghai, Xining were higher than others. CONCLUSION This method is stable, reliable, reproduceible. The experimental results supply the basic information for medicinal investigation and the product development of Pterocephalus hookeri.