玳玳果总黄酮体外抗氧化作用的研究

In Vitro Antioxidant Effect of the Total Flavones of Citrus Aurantium L. var Daidai Tanaka Fruits

关键词:  玳玳果  总黄酮  有效部位  抗氧化

Key words:  Citrus aurantium L.var daidai Tanaka fruits  total flavones  effective fraction  antioxidant effects

摘要:

目的 探讨玳玳果总黄酮有效部位的抗氧化作用。方法 分别采用二苯代苦味酰基自由基法(DPPH·法)、水杨酸法(Fenton反应法)和硫代巴比妥酸法(TBAS法)等方法，进行玳玳果总黄酮有效部位抗氧化药效实验研究，评价玳玳果总黄酮对DPPH·自由基、·OH自由基的清除能力，测定玳玳果总黄酮对小鼠肝脂质过氧化的抑制作用。结果　玳玳果总黄酮有效部位对DPPH·自由基及·OH自由基均具有良好的清除作用，其自由基清除能力以半数清除率计分别为0.417 mg·mL^-1和3.807 mg·mL^-1，同时对小鼠肝脂质过氧化具有显著的抑制作用，呈良好的量效相关性。结论　玳玳果总黄酮有效部位具有良好的抗氧化清除自由基作用。

Abstract:

OBJECTIVE To study the antioxidant effects of the total flavones of Citrus aurantium L. var daidai Tanaka fruits(TFEFD) in vitro. METHODS The scavenging effect of ·OH and DPPH· as well as the inhibitory effect of lipid peroxidation in mouse liver were measured by the salicylic acid, DPPH·, and thiobarbituric acid methods. RESULTS TFEFD could scavenge DPPH· and ·OH(IC₅₀ 0.417 mg·mL^-1 and 3.087 mg·mL^-1, respectively). It also significantly inhibited lipid peroxidation in mouse liver. There was a positive dose-effect relationship between the scavenging activity and the concentrations of TFEFD. CONCLUSION TFEFD has the capability to scavenge radicals and serve as one of the active natural antioxidants.