山芝麻水提取物对小鼠免疫性肝损伤的保护作用

Protective Effect of the Water Extract from Helicteres Angustifolia on Immunological Liver Injury in Mice

关键词:  山芝麻  免疫性肝损伤  T淋巴细胞亚群  刀豆蛋白A

Key words:  Helicteres angustifolia  hepatic damage  T lymphocyte subpopulation  concanavalin-A

摘要:

目的 观察山芝麻水提取物对小鼠免疫性肝损伤的保护作用。方法 将60只小鼠随机分为正常对照组、模型组、日达仙阳性对照组(4.2 μg·kg^-1·d^-1)，以及山芝麻高、中、低剂量组(20，10，5 g·kg^-1·d^-1)，各组预防性给药15 d，除正常对照组外，其余各组小鼠尾静脉注射20 mg·kg^-1刀豆蛋白A(Con A)。12 h后测定血清谷草转氨酶(AST)、谷丙转氨酶(ALT)的活性，流式细胞术测定全血中CD³₊、CD⁴₊、CD⁸₊T细胞亚群比率，ELISA方法测定血清肿瘤坏死因子(TNF-α)和γ干扰素(IFN-γ)的水平。结果 与模型组比较，10，20 μg·kg^-1·d^-1剂量的山芝麻能明显降低Con A介导的肝损伤小鼠血清中AST、ALT的水平，显著提高CD³₊、CD⁴₊、CD⁴₊/CD⁸₊比率，明显降低血清中炎性细胞因子IFN-γ和TNF-α的含量。结论 山芝麻水提取物对免疫性肝损伤具有保护作用，其机制可能与调整T细胞亚群的活性和减少炎性细胞因子的作用有关。

Abstract:

OBJECTIVE To observe the protective effect of the water extract from Helicteres angustifolia (WEHA) on immunological liver injury in mice. METHODS Sixty mice were randomly divided into normal group, model group，Zadaxi group (4.2 μg·kg^-1·d^-1), and high, medium, low-dose WEHA-treated group (20, 10, 5 g·kg^-1·d^-1). The normal and model groups were given normal saline orally, all mice in other groups were given the corresponding drugs. After 15 d, beside mice in normal group, other groups were injected with concanavalin A (Con A) 20 mg·kg^-1 via the tail vein to damage mice’s liver. Using automatic biochemical analyzer, the alanine aminotransferase(ALT) and aspartate aminotransferase (AST) in serum were examined. The percentage of T lymphocyte subsets CD³₊、CD⁴₊、CD⁸₊ cells were detected in plasma by a flow cytometer, using ELISA for determination of the serum cytokines: interferon-gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α). RESULTS Compared with model group, 10, 20 g·kg^-1·d^-1-dose WEHA could significantly decrease the activity of AST and ALT, increase percentage of CD³₊，CD⁴₊ cell and CD⁴₊/CD⁸₊. And WEHA also could significantly reduce the inflammatory cytokines IFN-γ and TNF-α level. CONCLUSION The water extract from Helicteres angustifolia is effective in protecting immunological liver injury in mice induced by Con A and this mechanism maybe closely associated with its action in regulating the T-cell subsets and reducing the inflammatory cytokines.