醉香含笑根皮挥发性成分的GC-MS分析及对NIH 3T3细胞生长抑制作用

GC-MS Analysis of Volatile Components from Roots of Michelia Macclurei Dandy. and Growth Inhibition of NIH 3T3 Cell Line

关键词:  醉香含笑根皮  挥发性成分  GC-MS分析  NIH 3T3细胞  生长抑制

Key words:  root extract of Michelia macclurei Dandy.  volatile components  GC-MS analysis  NIH 3T3 cell lines  growth inhibition

摘要:

目的 分析醉香含笑根皮的挥发性成分，并考察醉香含笑根皮提取物(REMMD)对小鼠成纤维细胞NIH 3T3的体外生长抑制作用。方法 水蒸气蒸馏法提取醉香含笑根皮的挥发性成分，用气相-质谱联用(GC-MS)技术分析，并用峰面积归一化法测定各成分相对含量；采用MTT法检测REMMD对NIH 3T3细胞体外生长抑制作用。结果 共检出57个色谱峰，确定了其中31种化合物，占该挥发性成分总量的63.31%。结论 REMMD的挥发性成分以 N,N’-二苯甲酰基-庚二胺为主(28.97%)，其他成分有棕榈酸及棕榈酸乙酯(7.03%)、高香草酸(3.25%)、4-[(1E)-3-羟基-1-丙烯基]-2-甲氧基-苯酚(3.16%)、木香烯内酯(2.33%)、糠醛及其衍生物(2.5%)。25，50，75，100 μg·mL^-1的REMMD能显着抑制NIH 3T3细胞的生长，其抑制率呈浓度依赖关系，提示其具有抗纤维化疾病的良好研究开发前景。

Abstract:

OBJECTIVE The volatile components from roots of Michelia macclurei Dandy. were analysed, and the inhibition effects of root extract of Michelia macclurei Dandy. (REMMD) on growth of NIH 3T3 cell lines in vitro were explored. METHODS The volatile components from root of Michelia macclurei Dandy. were extracted with steam distillation method and analysed by GC-MS. The relative content of each component was calculated by peak area normalization. The inhibiting effects of REMMD on growth of NIH 3T3 cell lines in vitro were examined by MTT assay. RESULTS Fifty seven peaks were detected, in which 31 compounds were identified, which made up 63.31% of the total volatile components. CONCLUSION The main component in the volatile components is N, N’-di-benzoyloxy-heptanediamide(28.97%), other components are palmitic acid and palmitic acid ethyl ester(7.03%), 4-hydroxy-3-methoxy phenyl acetic acid (3.25%), 4-[(1E)-3- hydroxy-1-propenyl]-2- methoxyphenol(3.16%), costunolide(2.33%), furaldehyde and its derivatives(2.5%). REMMD with concentrations of 25, 50, 75, 100 μg·mL^?1 could significantly inhibit the growth of NIH 3T3 cell lines in vitro, therefore the results are enlightening for good researching and developing perspects of anti-fibrosis diseases.