| 摘要: |
| 目的 建立高效液相色谱法同时测定石榴健胃丸中桂皮醛、胡椒碱含量的方法。方法 色谱柱采用CAPCELL PAK C18( 4.6 mm×250 mm，5 mm)柱；流动相为乙腈-水(40∶60)；流速：1.0 mL·min-1；检测波长：290 nm；柱温：30 ℃。结果 桂皮醛在1.149 5～13.793 5 μg之间呈良好的线性关系，平均回收率为97.27%，RSD为1.39%(n=6)；胡椒碱在0.726 4～2.724 μg之间呈良好的线性关系，平均回收率为96.51%，RSD为1.39%。结论 本法简便、准确，专属性强，可作为控制石榴健胃丸质量的方法。 |
| 关键词: 石榴健胃丸 桂皮醛 胡椒碱 高效液相色谱法 |
| DOI： |
| 分类号: |
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| Determination of Cinnamaldehyde and Piperine in Shiliu Jianwei Pill by HPLC |
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NI Lin, YANG Xi
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Gansu Provincial Institute of Food and Drug Control, Gansu Province Traditional Chinese Medicine Quality and Safety Evaluation of Engineering Technology Research Center, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To determine the content of Cinnamaldehyde and Piperine in Shiliu jianwei Pill. METHODS The analysis was performed on a CAPCELL PAK C18 Column(4.6 mm×250 mm, 5 mm)at 30℃ with acetonitrile-water (40∶60)as the mobile phase and the flow rate of 1.0 mL·min-1. The detection wavelength was 290nm. RESULITS Cinnamaldehyde have good linearity in the range of 1.149 5-13.793 5 μg(r=0.999 7). The average recovery was 97.27% and RSD was 1.39% (n=6). Piperine have good linearity in the range of 0.726 4-2.724 μg, (r=0.999 1) respectively. The average recovery was 96.51% and RSD was 1.39% (n=6). CONCLUSION The established method is accurate, reliable and specific. The quality of the pill can be controlled by the method. |
| Key words: Shiliu Jianwei Pill Cinnamaldehyde Piperine HPLC |
