四川攀西地区五色梅中b-榄香烯的含量分析

Content Analysis on b-elemene in Lantana camara Linn in Panxi Area of Sichuan

关键词:  五色梅  b-榄香烯  含量测定  高效液相色谱法  分布规律

Key words:  Lantana camara Linn  b-elemene  content  HPLC  distributing

摘要:

目的 建立五色梅中b-榄香烯的含量分析方法，并对四川攀西地区恶性杂草植物五色梅中的b-榄香烯含量和分布规律进行测定分析。方法 采用HPLC测定五色梅样品中b-榄香烯含量，Eclipse XDB-C18色谱柱(4.6 mm×150 mm，5 mm)，流动相：乙醇-乙腈-水=70︰10︰20，流速：1.0 mL·min^-1，检测波长：210 nm，进样量：20 μL，柱温：30 ℃。用TLC和紫外光谱对样品中的b-榄香烯进行鉴定分析。 结果 HPLC测b-榄香烯含量在0.007 5～0.120 0 mg·mL^-1内线性关系良好(r=0.999 5)，平均加样回收率为99.3%。五色梅叶和花中均含b-榄香烯，而以叶中含量最高，鲜叶与花中含量分别为0.025%和0.007%。鲜叶和自然晒干叶中b-榄香烯含量差异无统计学意义，但均显著高于60 ℃烘干叶。结论 本法适用于五色梅中b-榄香烯的含量测定，本研究可为开辟新的b-榄香烯来源、五色梅的开发利用和治理提供参考。

Abstract:

To establish a method to determine and study the content of b-elemene in Lantana camara Linn in Panxi area of Sichuan. METHODS The content of b-elemene was determined by HPLC with an Eclipse XDB-C₁₈ (4.6 mm×150 mm，5 mm) column, mobile phase EtOH-CAN-H₂O (70︰10︰20), flow rate 1.0 mL·min^-1, and UV detection wavelength 210 nm. The qualitative analysis of b-elemene was conducted by TLC and UV spectrum. RESUALTS A good linear relationship at the range of 0.007 5-0.120 0 mg·mL^-1(r=0.999 5) was obtained and the average recovery was 98.02%. b-elemene was tested out in leaves and flowers of Lantana camara Linn and not found in their roots, stems and fruits. The fresh leaves and naturally dried leaves had the highest content, which were higher notably than that of 60 ℃ dried leaves. The b-elemene contents in fresh leaves and flowers were 0.025% and 0.007%, respectively. CONCLUSION The mothod is suitable for b-elemene determination of Lantana camara Linn. The experiment can be used as references to find new resource of b-elemene and utilize Lantana camara Linn.