中药橡胶膏剂基质辅料过敏性考察

Study the Allergy of Substrate Materials in TCM Adhesive Plasters

关键词:  橡胶膏剂  基质辅料  致敏性

Key words:  adhesive plasters  adjuvant material  allergenic

摘要:

目的 研究中药橡胶膏剂基质辅料的潜在致敏情况，为橡胶膏剂基质辅料安全性评价提供依据。方法 建立小鼠接触性过敏反应模型，检测各辅料对小鼠耳肿胀的影响。同时，采用 ELISA 法检测小鼠血清中白细胞介素-4(IL-4)、γ-干扰素(IFN-γ)及可溶性白细胞介素-2受体(sIL-2R)水平。通过比较空白组与致敏组、不同辅料组以及由不同辅料按一定配比制成的空白基质组小鼠之间左右耳厚度差、重量差和细胞因子的变化，探讨橡胶膏剂中各基质辅料的致敏情况。结果 与空白组比较，致敏组、橡胶组和松香组小鼠耳厚度差、重量差以及血清中IL-4、IFN-γ、sIL-2R水平差异有统计学意义；氧化锌、二氧化钛和羊毛脂组差异无统计学意义；不同橡胶组和松香组之间以及由不同橡胶和松香制成的空白基质组之间比较，小鼠耳厚度差、重量差以及血清中IL-4、IFN-γ、sIL-2R水平有显著性差异。结论 橡胶膏剂主要基质辅料中橡胶和松香有较高的致敏性，氧化锌、二氧化钛和羊毛脂的致敏性相对较低；且 5 号胶的致敏性高于越南胶，普通松香的致敏性高于纯化松香；由 5 号胶、普通松香、氧化锌、羊毛脂按一定的组方配比制成的空白基质的致敏性高于由越南胶、纯化松香、氧化锌、羊毛脂按同样比例制成的空白基质。

Abstract:

OBJECTIVE To investigate the potential allergy of substrate materials in TCM adhesive plasters and provide the basis safety evaluation of adjuvant materials in TCM adhesive plasters. METHODS Establish mice model of allergy contact dermatitis, detect each substrate materials for mice auricular swelling effect, adopt ELISA test to detect the content of IL-4, IFN-γ, sIL-2R. By comparing the ears thickness, weight, cytokines in adjuvant material groups, blank group, positive control group and different blank matrix groups, the potential allergy of adjuvant material in TCM adhesive plasters was investigated. RESULTS The ears thickness, weight, cytokines from positive control group, rubber group, rosin group were all different from blank group, but there was no significant differences in Zinc oxide, TiO₂ and lanolin group. CONCLUSION The allergy of rubber and rosin are higher than zinc oxide, TiO₂ and lanolin.