HPLC测定铁皮枫斗颗粒中甘露糖的含量

Determination of Mannose in TiePiFengDou Granules by HPLC

关键词:  甘露糖  铁皮枫斗颗粒  高效液相色谱法

Key words:  mannose  TiePiFengDou granules  HPLC

摘要:

目的 建立铁皮枫斗颗粒中铁皮石斛多糖水解产物甘露糖的含量测定方法。方法 采用HPLC，色谱柱采用Agilent Eclipse XDB-C₁₈柱(250 mm×4.6 mm，5 μm)；流动相为乙腈-0.02 moL·L^-1的乙酸铵溶液(20∶80)；流速：1.0 mL·min^-1；柱温：30 ℃；检测波长：250 nm。结果 甘露糖进样量在80.64~403.20 μg内呈良好的线性关系(r=0.999 8)，平均加样回收率为98.52%，RSD为1.35%(n=6)。结论 该方法简便可靠，结果稳定，重复性好，可准确测定铁皮枫斗颗粒中甘露糖的含量，且该方法较以前测定人参皂苷Rb1的方法更加合理，可用于铁皮枫斗颗粒的质量控制。

Abstract:

OBJECTIVE The HPLC method for determination of Mannose in TiePiFengDou granules was established. METHODS In this method, Agilent Eclipse XDB-C₁₈ column(250 mm × 4.6 mm, 5 μm) was used. Mobile phase consisted of acetonitrile-0.02 mol·L^-1 of ammonium acetate (20∶80). Flow rate was 1.0 mL·min^-1, and detection wavelength was 250 nm. RESULTS Mannose had a good linearity within the range of 80.64-403.20 μg. The average recovery was 98.52%, RSD= 1.35%(n=6). CONCLUSION The method is accurate, convenient and reproducible. Results are stable. The method can be applied for accurately determination of mannose in TiePiFengDou granules and it is more reasonable than the determination of ginsenoside Rb1as quality standard. The method can be used as the quality control of TiePiFengDou.