超临界CO2萃取远志挥发油气相指纹图谱对比研究

Supercritical CO2 Extraction of Volatile Components of Polygala Tenuifolia Willd. and Analysis of Characteristics through Fingerprint

关键词:  远志  挥发油  超临界CO₂  气相色谱指纹图谱

Key words:  Polygala tenuifolia Willd.  volatile oil  supercritical CO₂ extraction  GC fingerprint

摘要:

目的 建立远志挥发油成分的气相指纹图谱，研究种植与野生、不同提取方法对远志挥发油成分的影响。方法 采用超临界CO₂萃取对10批不同产地的远志提取挥发油，用气相色谱仪对挥发油进行分析，建立超临界CO₂远志挥发油的气相指纹图谱。结果 超临界CO₂萃取远志挥发油出油率在0.29%~0.82%之间，高于水蒸气蒸馏法提取率，与水蒸气蒸馏法提取法得到的易挥发油成分基本相同，人工种植较野生远志挥发油成分种类更丰富。结论 超临界CO₂萃取法提取远志挥发油效率高，为研究远志挥发性成分提供了较好的提取方法。

Abstract:

OBJECTIVE To study the effects of cultivated growing and wild growing Polygala tenuifolia Willd specieses and different extracted methods on volatile oil of Polygala tenuifolia Willd, characteristics of fingerprints was set up based on supercritical CO₂ extraction method. METHODS The volatile oil of Polygala tenuifolia Willd of different origins was refined through the supercritical CO₂ extraction, and was analyzed by GC fingerprint. RESULTS The extraction rate of volatile oil from Polygala tenuifolia Willd through the supercritical CO₂ extraction was between 0.29%-0.82%, and the components of volatile oil were basically the same compared with those extrated by steam distillation; the type of the volatile oil in cultivation was more abundant compared with the components of volatile oil in wild Polygala tenuifolia Willd. CONCLUSION The method of the supercritical CO₂ extraction is high efficiency, which provides a better extraction method for the volatile oil of Polygala tenuifolia Willd.