| 引用本文: | 韦英杰，姜金生，谭晓斌，陈斌，刘炜，胡春萍，马世平，贾晓斌.HPLC-ESI/MS分析5-羟基黄酮在大鼠体内的代谢产物[J].中国现代应用药学,2012,29(1):60-63. |
| WEI Yingjie, JIANG Jinsheng, TAN Xiaobin, CHEN Bin, LIU Wei, HU Chunping, MA Shiping, JIA Xiaobin.Metabolites Analysis of 5-Hydroxyflavone in Rat by HPLC-ESI/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(1):60-63. |
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| 摘要: |
| 目的 研究5-羟基黄酮在大鼠体内的代谢产物。方法 应用高效液相色谱-电喷雾质谱(HPLC-ESI/MS)检测大鼠灌胃5-羟基黄酮后血浆、尿液、胆汁和粪便中的代谢产物。实验采用Zorbax C18色谱柱，0.05%甲酸乙腈-0.05%甲酸水二元线性梯度洗脱进行色谱分离，并与电喷雾质谱联用，根据正离子模式的分子离子峰获得化合物分子量信息，推测化合物的可能结构。结果 仅在大鼠粪便中检测到原形成分，在大鼠尿液、粪便、血浆、胆汁中检测到5-羟基黄酮葡糖醛酸结合物。结论 5-羟基黄酮吸收差，在大鼠体内主要以II相代谢产物葡萄糖醛酸结合物的形式存在。 |
| 关键词: 5-羟基黄酮 代谢 大鼠 高效液相色谱-电喷雾质谱 |
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| 基金项目:国家自然科学基金资助项目(30973978) |
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| Metabolites Analysis of 5-Hydroxyflavone in Rat by HPLC-ESI/MS |
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WEI Yingjie, JIANG Jinsheng, TAN Xiaobin, CHEN Bin, LIU Wei, HU Chunping, MA Shiping, JIA Xiaobin1,2
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1.Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China;2.Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China
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| Abstract: |
| OBJECTIVE To study the metabolites of 5-hydroxyflavone in rat. METHODS High performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) was used for analysis of metabolites of 5-hydroxyflavone in rat after oral administration. The separation was performed with a Zorbax C18 column using a binary gradient elution of 0.05% formic acid acetonitrile-0.05% formic acid water. The quasi-molecular ions of compounds in positive mode were observed for molecule mass information, and the potential structures were presumed. RESULTS Parent component 5-hydroxyflavone was found only in rat fence, while glucuronide conjugates of 5-hydroxyflavone were found in rat urine, fence, plasma and bile. CONCLUSION Absorption of 5-hydroxyflavone is poor, and the major forms of 5-hydroxyflavone in rat are metabolites of phase II glucuronide conjugates. |
| Key words: 5-hydroxyflavone metabolism rat LC-ESI/MS |
