生脉注射液对青春期大鼠睾丸扭转复位后Bcl-2/Bax蛋白表达的影响

Effects of Shengmai Injection on the Expression of Bcl-2 and Bax Following Testicular Torsion/Detorsion in Adolescent Rats

关键词:  生脉注射液  睾丸扭转  Bcl-2/Bax  青春期大鼠

Key words:  Shengmai injection  testicular torsion  Bcl-2/ Bax  adolescent rat

摘要:

目的 探讨生脉注射液对青春期大鼠睾丸扭转复位后Bcl-2/Bax蛋白表达的影响。方法 6周龄健康SD大鼠，♂，16只，建立单侧睾丸扭转复位模型。随机分为睾丸扭转复位生理盐水(对照)组和睾丸扭转复位生脉注射液(实验)组，每组8只。术后9周取双侧睾丸标本，HE染色后观察睾丸组织形态改变；免疫组织化学ABC法检测Bcl-2/Bax蛋白在睾丸组织中的表达。结果 实验组生精小管管壁完整，生精上皮排列规则，细胞层次清晰；对照组生精小管管壁不完整，生精上皮变薄，细胞排列紊乱，层次减少，部分细胞可见核固缩，核碎裂崩解，可见多核细胞。与对照组相比，实验组睾丸组织Bcl-2蛋白表达增加(P<0.05)，Bax蛋白表达减少(P<0.05)。结论 生脉注射液可能通过增加Bcl-2蛋白和降低Bax蛋白的表达减轻细胞凋亡，对睾丸扭转复位后的损伤有保护作用。

Abstract:

OBJECTIVE To investigate the effect of Shengmai injection extract on the expression of Bcl-2 and Bax following testicular torsion/detorsion in adolescent rats. METHODS Sixteen healthy adolescent male SD rats(6 weeks)were randomly divided into experimental group(testicular torsion/detorsion plus Shengmai injection extract, n=8) and control group(testicular torsion/detorsion plus saline, n=8). Animals were submitted to unilateral 720o testicular torsion,then detorsion was done in two hours. Nine weeks later, the expression of Bcl-2 and Bax were detected by immunohistochemical method. RESULTS In experimental group, the seminiferous tubule was complete, the spermatogenic epithelium arranged regularly and the levels of cells were more clear compared with those in the control group. There was some phenomenon such as pyknosis, karyorrhexis appearing in the cells of control group. In the torsed testes, the apoptosis index of germ cell and Bax expression significantly increased compared with that in the control group (P<0.05) while Bcl-2 expression obviously decreased (P<0.05). The apoptotic cells were mostly pechytene spermatocytes and round spermatides. CONCLUSION Shengmai injection can obviously increase the expression of Bcl-2 while decrease the expression of Bax to inhibit the apoptosis.