迷迭香中鼠尾草酸的抗MRSA活性研究

Anti-MRSA Activity of Carnosic Acid in Rosemary

关键词:  迷迭香  鼠尾草酸  抗MRSA  相加作用  协同作用

Key words:  Rosemarinus officinalis  carnosic acid  anti-MRSA  additive effect  synergism

摘要:

目的 对迷迭香中鼠尾草酸的抗MRSA(耐甲氧西林金黄色葡萄球菌)活性进行研究。方法 采用硅胶柱层析法对迷迭香提取物(鼠尾草酸含量72.4%)进行分离精制获得鼠尾草酸，然后以标准菌株MRSA ATCC 33592和临床分离菌株MRSA 01~08为指示菌，采用微量肉汤稀释法测定鼠尾草酸对MRSA菌株的最低抑菌浓度和最低杀菌浓度；运用棋盘格法设计试验，采用微量肉汤稀释法，分别测定鼠尾草酸与苯唑西林钠或氨苄西林钠的联合抗MRSA作用。结果 分离精制获得含量为98.6%的鼠尾草酸，其对指示菌MRSA ATCC33592和MRSA 01~08的最低抑菌浓度的范围为8~16 μg·mL^-1，最低杀菌浓度均为32 μg·mL^-1；与苯唑西林钠或氨苄西林钠合用对MRSA指示菌的部分抑菌浓度指数(FICIs)：0.5≤FICIs<1.0。结论 鼠尾草酸具有明显的抗MRSA活性，与苯唑西林钠或氨苄西林钠合用时呈现抗MRSA的相加或协同作用。

Abstract:

OBJECTIVE To study the anti-MRSA (anti-methicillin-resistant Staphylococcus aureus) activity of carnosic acid isolated from Rosmarinus officinalis L.. METHODS Carnosic acid was purified from rosemary extract (contained 72.4% carnosic acid) by silica gel chromatography. Against MRSA ATCC 33592 and eight clinic isolates of MRSA 01 to 08, the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of carnosic acid were tested by broth microdilution method, and the anti-MRSA effect of carnosic acid combined with oxacillin sodium or ampicillin sodium was designed with checkerboard method and determined by broth microdilution method. RESULTS The content of carnosic acid purified was 98.6%. Against MRSA ATCC 33592 and eight clinnic isolates of MRSA 01 to 08, the MICs range and MBCs were respectively 8-16 μg·mL^-1 and 32 μg·mL^-1, and the fractional inhibitory concentration indices (FICIs) of carnosic acid combined with oxacillin sodium or ampicillin sodium were 0.5 or 0.5-1.0. CONCLUSION Carnosic acid shows obvious anti-MRSA actvitiy, and the anti-MRSA effect of carnosic acid combined with oxacillin sodium or ampicillin sodium is synergistic or additive.