中药莪术对大鼠十二指肠平滑肌收缩的影响

Effect of Curcumae Rhizoma on Rat Duodenum Smooth Muscle Contraction

关键词:  莪术  十二指肠  促进  机制

Key words:  Curcumae Rhizoma  smooth muscle  facilitate  mechanisms

摘要:

目的 探讨中药莪术(Curcumna Rhizoma)水提液对大鼠离体十二指肠平滑肌收缩运动的影响，并初步探索其作用机制。方法 制作大鼠离体十二指肠标本，用恒温灌流的方法，使用BL-420生物机能实验系统观察不同浓度莪术对大鼠离体十二指肠平滑肌的收缩效应。选适当终浓度莪术分别与阿托品、去甲肾上腺素、异丙肾上腺素、维拉帕米共同孵育，观察莪术对它们引起平滑肌收缩作用的影响；制作不同浓度新斯的明肠管收缩作用的量效曲线，与莪术组进行对比，初步探讨其可能的作用机制，并作胆碱酯酶活性测定辅助证明是否与新斯的明作用机制相似。结果 莪术可以对大鼠离体十二指肠的收缩有兴奋作用，呈剂量依赖性；莪术可以部分被M受体阻断药阿托品阻断、β受体激动药异丙肾上腺素和钙通道阻滞剂维拉帕米对肠管收缩的抑制作用；对α受体激动药去甲肾上腺素无影响；与乙酰胆碱酯酶抑制剂新斯的明的作用相似。结论 莪术明显促进大鼠离体十二指肠平滑肌收缩作用，呈剂量依赖性。其作用机制可能是通过抑制胆碱酯酶，与M受体介导有关，同时还可能与抑制α和β受体有关。

Abstract:

OBJECTIVE To investigate the effect Curcuma Rhizoma on rat duodenum smooth muscle contraction and its mechanisms. METHODS After isolated rat duodenum samples were prepared, it was observed that the effect of Curcuma Rhizoma at different concentrations on rat duodenum smooth muscle contraction using BL-420 biological and functional experimental system with constant perfusion way. It was observed that the effect of Curcuma Rhizoma at different optimum concentrations on smooth muscle contractile amplitudes induced by atropine, norepinephrine, isoproterenol, verapamil respectively. Dose-effect curve of different concentrations of neostigmine on intestinal contraction was recorded, comparing with that of Curcuma Rhizoma group. Cholinesterase activity was detected so as to assisting the proof that whether its one of mechanisms is similar to that of neostigmine. RESULTS Curcuma Rhizoma facilitated significantly rat duodenum smooth muscle contraction in a dose-dependent manner; Curcuma Rhizoma antagonized partly the inhibitory effect on intesinal smooth muscle induced by a M receptor blocker(atropine), β receptor agitator(isoprenaline), calcium channel blocker(verapamil). However, it did not act on α receptor(norepinephrine). Its action was similar to that of the acetylcholin esterase activity which was inhibited by acetylcholin esterase inhibitor(neostigmine). CONCLUSION The mechanisms of its effect may be mediated by M receptors by inhibiting cholinesterase. It also may be related to inhibition of α and β receptor.