姜黄素对急性肺损伤小鼠肺组织GSH-PX和iNOS活性的影响

Effect of Curcumin on Acute Lung Injury of Mice with GSH-PX and iNOS Activity in Lung Tissues

关键词:  急性肺损伤  姜黄素  谷胱甘肽过氧化物酶  诱导型一氧化氮合酶

Key words:  acute lung injury  curcumin  glutathione peroxidase  inducible nitric oxide synthase

摘要:

目的 探讨姜黄素对博莱霉素(BLM)诱导的急性肺损伤(Acute lung injury，ALI)小鼠肺组织谷胱甘肽过氧化物酶(GSH-PX)、诱导型一氧化氮合酶(iNOS)活性的影响。方法 博莱霉素诱导小鼠急性肺损伤模型，光镜观察肺组织学改变，测定GSH-PX、iNOS活性变化。结果 姜黄素能降低肺组织中iNOS活性，提高小鼠体内GSH-PX活性，与假手术组相比，第3、7天时模型组iNOS活性明显增高(P<0.01)，姜黄素中、高剂量组和模型组相比，iNOS活性下降显著(P<0.01)；与假手术组相比，第3、7天时模型组GSH-PX活性明显减少(P<0.01)，姜黄素中、高剂量组和模型组相比，GSH-PX活性显著增加(P<0.01)。病理组织学检查亦表明，一定剂量的姜黄素可明显减轻肺组织损伤的程度。结论 一定剂量姜黄素在BLM诱导的急性肺损伤中有一定的防治作用。其机制可能是通过升高肺组织的GSH-PX活性, 降低iNOS的活性，重建机体氧化、抗氧化平衡有关。

Abstract:

OBJECTIVE To investigate the effects of curcumin on GSH-PX, iNOS activity in lung tissues in acute lung injury (ALI) mice induced by bleomycin (BLM). METHODS BLM was injected intravenously into mice to establish the ALI model. The histological changes were observed with microscope. The changes of GSH-PX and iNOS were measured to reveal the acute lung injury. RESULTS Curcumin was able to increase the activity of GSH-PX，reduce the activity of iNOS. Compared with the sham group, iNOS in model group were significantly higher (P<0.01) at the 3rd and 7th day. In high-dose and medium-dose group of curcumin, iNOS decreased significantly (P<0.01) compared with model group. GSH-PX decreased significantly (P<0.01) in model group at 3rd and 7th day. In high-dose and medium-dose group, GSH-PX activity increased significantly (P<0.01) compared with model group. The lung pathological manifestations also displayed obvious pathological improvement. CONCLUSION Curcumin has protective effect at the early phase of ALI. The mechanism may be the increase of lung tissue GSH-PX activity, inhibited activity of iNOS as well as the balance between reconstruction oxidative and anti-oxidant.