| 引用本文: | 封淑华，韩桂茹，赵志军.清胃黄连丸快速TLC鉴别与小檗碱新荧光扫描定量[J].中国现代应用药学,2012,29(6):524-529. |
| FENG Shuhua, HAN Guiru, ZHAO Zhijun.Fast Identification by TLC and Quantitative Determination by Fluorescence TLCS for Qingwei Huanglian Pills[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(6):524-529. |
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| 摘要: |
| 目的 建立清胃黄连丸的快速TLC鉴别及小檗碱的高灵敏度荧光扫描定量方法。方法 采用同一供试品溶液在2块薄层板上鉴别黄芩、牡丹皮、连翘、栀子和甘草，用荧光扫描法测定了清胃黄连丸中盐酸小檗碱的含量。结果 盐酸小檗碱点样量在0.019 68~0.098 4 μg内呈良好的线性关系。盐酸小檗碱平均回收率为99.41%，RSD为1.34%(n=9)。结论 TLC鉴别斑点清晰，阴性无干扰。清胃黄连丸的快速TLC鉴别与小檗碱新荧光扫描定量，简便、快捷、准确、实用，有效提高了传统丸剂的质量检测速度。 |
| 关键词: 清胃黄连丸 快速薄层色谱法鉴别 盐酸小檗碱 荧光扫描 |
| DOI： |
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| 基金项目:国家药典委员会中国药典2010年版课题(ZH-434，ZH-435) |
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| Fast Identification by TLC and Quantitative Determination by Fluorescence TLCS for Qingwei Huanglian Pills |
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FENG Shuhua, HAN Guiru, ZHAO Zhijun
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Hebei Provincial Institute for Drug control, Shijiazhuang 050011, China
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| Abstract: |
| OBJECTIVE To establish a method for fast identification by TLC and quantitative determination by fluorescence TLCS for Qingwei Huanglian pills. METHODS Scutellariae Radix, Moutan Coxtex, Forsythiae Fructus, Gardeniae Fructus, Glycyrrhizae Radix et Rhizoma were identified by TLC, using the same sample solution in two TLC plate. The content of berberine hydrochloride in Qingwei Huanglian pills was determined by fluorescence TLCS. RESULTS The methodological study showed a good linear correlation in the range from 0.019 68 to 0.098 4 μg of the berberine hydrochloride. The average recovery of berberine hydrochloride (n=9) was 99.41%, and RSD was 1.34%. CONCLUSION The method is simple, accurate, repeatable and with good precision. The method can be used for quality control of Qingwei Huanglian pills. The detection speed was effectively improved for assay of traditional pills. |
| Key words: Qingwei Huanglian pills fast identification by TLC berberine hydrochloride fluorescence TLCS |
