| 引用本文: | 赵奇江，李芬芬，董新威，徐哲荣，杨云梅，谢强敏.他汀类药物对TNFa诱导成骨细胞生长抑制的保护作用[J].中国现代应用药学,2012,29(5):389-393. |
| ZHAO Qijiang, LI Fenfen, DONG Xinwei, XU Zherong, YANG Yunmei, XIE Qiangmin.Protective Effects of Statins on TNFα-induced Growth Inhibition in MC3T3-E1 Osteoblastic Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(5):389-393. |
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| 摘要: |
| 目的 比较4种他汀类药物对TNF-α诱导的小鼠成骨细胞(MC3T32-E1)生长抑制的影响。方法 小鼠成骨细胞MC3T3-E1用DMEM+10%胎牛血清培养，细胞活性用MTT法测定。结果 TNFα (1~100 ng·mL-1)呈浓度和时间依赖抑制MC3T3-E1细胞生长；低浓度的辛伐他汀(10-10~10-7 mol·L-1)、氟伐他汀(10-10~10-6 mol·L-1)和阿托伐他汀(10-8~10-6 mol·L-1)处理MC3T3-E1细胞72 h后能明显促进成骨细胞生长，而低浓度的罗舒伐他汀(10-10~10-7 mol·L-1)无作用，在高浓度(10-6~10-5 mol·L-1)时抑制MC3T3-E1的生长；用辛伐他汀、氟伐他汀和阿托伐他汀与TNF-α(10 ng·mL-1)共同培养MC3T3-E1细胞72 h，呈浓度依赖逆转TNF-α诱导的MC3T3-E1生长抑制，而罗舒伐他汀在低浓度时显示较强的逆转作用，在高浓度(10-6 mol·L-1)时无作用或促进TNFα诱导的MC3T3-E1生长抑制。结论 辛伐他汀、氟伐他汀和阿托伐他汀(10-10~10-7 mol·L-1)能促进MC3T3-E1细胞生长，逆转TNFα诱导的MC3T3-E1生长抑制；而罗舒伐他汀高浓度(10-6~10-5 mol·L-1)时抑制MC3T3-E1的生长，在低浓度时能明显逆转TNF-α诱导的成骨细胞生长抑制。 |
| 关键词: 骨质疏松症 成骨细胞 他汀类 肿瘤坏死因子α |
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| 基金项目:国家自然科学基金资助项目(30973542) |
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| Protective Effects of Statins on TNFα-induced Growth Inhibition in MC3T3-E1 Osteoblastic Cells |
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ZHAO Qijiang, LI Fenfen, DONG Xinwei, XU Zherong, YANG Yunmei, XIE Qiangmin1,2,3
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1.Dongpu Town of Community Health Service Center, Shaoxing 312069, China;2.Department of Pharmacology, Medical College of Zhejiang University, Hangzhou 310058, China;3.Department of Geriatrics, the First Affilated Hospital of Zhejiang University, Hangzhou 310003, China
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| Abstract: |
| OBJECTIVE To study if four statins could attenuate TNFα-induced growth inhibition and compare their effects in MC3T3-E1 osteoblastic cells. METHODS Murine osteoblastic MC3T3-E1 cells were cultured in DMEM essential medium supplemented with 10% fetal bovine serum and antibiotics. 3-(4,5-Dimethylthiazol-2yl-)-2,5-diphenyl tetrazolium bromide (MTT) was used to evaluate cell viability. RESULTS TNFα at concentrations (1-100 ng·mL-1) inhibited the viability of MC3T3-E1 cells in time and concentration-dependent manners. MC3T3-E1 cells exposed to low concentrations of simvastatin(10-10-10-7 mol·L-1), fluvastatin(10-10-10-6 mol·L-1) and atorvastatin(10-8-10-6 mol·L-1) increased growth and viability. In contrast, rosuvastatin(10-10-10-7 mol·L-1) had no effect, and high concentration of rosuvastatin(10-6-10-5 mol·L-1) decreased cell growth. MC3T3-E1 cells treated with simvastatin, fluvastatin and atorvastatin in the presence of TNFα concentration-dependently increased cell growth compared with that of untreated control cells. Low concentration of rosuvastatin(10-10-10-8 mol·L-1) in the presence of TNFα markedly increased cell growth, and high concentration of rosuvastatin(10-6 mol·L-1) in the presence of TNFα decreased cell growth. CONCLUSION The results demonstrate that statins can increase growth and viability of osteoblastic cells and decrease TNFα induced growth inhibition in murine osteoblastic MC3T3-E1 cells, and rosuvastatin at low and high concentration had opposite effects. |
| Key words: osteoporosis osteoblastic cell statins TNFα |
