粉防己碱对人膀胱移行细胞癌BIU-87细胞裸鼠移植瘤的实验研究

Effect of TET on xenografted tumor of human bladder cancer BIU-87 cells on nude mice

关键词:  粉防己碱  膀胱癌  Bcl-2  Bax  survivin  capseae-3

Key words:  tetrandrine  bladder cancer  Bcl-2  Bax  survivin  capseae-3

摘要:

目的 研究粉防己碱(TET)对人膀胱移行细胞癌BIU-87细胞株裸鼠移植瘤的作用机制。方法 14只裸鼠右后腿背侧皮下接种BIU-87移植瘤细胞后随机分为磷酸盐缓冲液(PBS)对照组(7只)和25 μg·mL^-1 TET治疗组(7只)。接种1个月后开始，每3 d一次于肿瘤局部注射给予PBS或TET，每3 d测量1次肿瘤大小；RT-PCR检测Bcl-2、Bax mRNA表达；Western-blot分析survivin、capseae-3蛋白表达。结果 TET对人膀胱癌BIU-87细胞裸鼠移植瘤的生长具有抑制作用，抑制率达66.6%。RT-PCR检测结果显示，与PBS对照组相比，TET能明显下调移植瘤Bcl-2 mRNA的表达，上调Bax mRNA的表达(P<0.05)；Western-blot结果显示经TET处理后survivin蛋白出现明显下调，而capseae-3蛋白的表达上调明显(P<0.05)。结论 TET能够抑制人膀胱癌BIU-87细胞裸鼠移植瘤的生长，其作用的分子机制与下调Bcl-2、survivin和上调Bax、capseae-3基因表达有关。

Abstract:

OBJECTIVE To investigate the effects of tetrandrine(TET) on the xenografted tumor of the human bladder cancer BIU-87 cell line on nude mice. METHODS Fourteen nude mice which had been inoculated with BIU-87 cells by subcutaneous injection into the right legs were divided into the PBS control group(7 mice) and the 25 μg·mL^-1 TET group(7 mice). After a month of transplantating tumor cell, mice were given PBS or TET into the tumor once every 3 days. Tumor volumes were measured every 3 days. RT-PCR was used to detect Bcl-2 and Bax mRNA expressions. The expressions of survivin and capseae-3 were detected by western-blot. RESULTS TET could inhibite the growth of xenografted tumor of human bladder cancer BIU-87 cells on nude mice, the inhibitory rate was 66.6%. RT-PCR analysis showed that TET significantly reduced tumor Bcl-2 mRNA expression and increased Bax mRNA expression (P<0.05); Western-blot showed that after TET treatment, the survivin protein markedly dropped, and capseae-3 protein expression increased significantly (P<0.05). CONCLUSION TET can inhibite the growth of xenografted tumor of human bladder cancer BIU-87 cells on nude mice. The molecular mechanism may be reducing the expressions of Bcl-2, survivin, and increasing the expression of Bax, capseae-3.