| 引用本文: | 袁干军，李沛波，潘微薇.两株特境链霉菌产抗MRSA次级代谢产物结构类型的快速鉴定[J].中国现代应用药学,2012,29(4):337-341. |
| YUAN Ganjun, LI Peibo, PAN Weiwei.Quick Identification of Anti-MRSA Secondary Metabolites Produced by Two Streptomyces Strains Isolated from Special Surroundings[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(4):337-341. |
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| 摘要: |
| 目的 对链霉菌ZZ027和ZZ021所产抗耐甲氧西林金黄色葡萄球菌(MRSA)次级代谢产物的结构类型进行快速鉴定。方法 用2A培养基对两链霉菌菌株进行发酵；再采用HPLC微分离技术和琼脂扩散法对抗MRSA色谱峰进行定位，然后采用TLC对发酵液中抗MRSA次级代谢产物进行分离与制备，所得抗MRSA组分经薄层显色、IR和13C-NMR谱测试分析，鉴定其结构类型。结果 链霉菌ZZ027和ZZ021抗MRSA组分的薄层显色、IR和13C-NMR谱特征均与大环内酯类化合物的相关表征一致。结论 链霉菌ZZ027和ZZ021所产抗MRSA次级代谢产物为大环内酯类化合物。 |
| 关键词: 链霉菌ZZ027 链霉菌ZZ021 大环内酯 结构类型 鉴定 |
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| 基金项目:江西农业大学博士启动基金(09003267) |
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| Quick Identification of Anti-MRSA Secondary Metabolites Produced by Two Streptomyces Strains Isolated from Special Surroundings |
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YUAN Ganjun, LI Peibo, PAN Weiwei1,2
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1.College of Bioscience and Engineering, Jiangxi Agricultral University, Nanchang 330045, China;2.School of Life Science, Sun Yat-sen University, Guangzhou 510275, China
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| Abstract: |
| OBJECTIVE To quickly identify the structure types of anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) secondary metabolites produced by Streptomyces sp. ZZ027 and Streptomyces sp. ZZ021. METHODS Two strains were fermented with 2A medium, and anti-MRSA chromatographic peaks were localized by HPLC micro-separation technology and agar diffusion method; anti-MRSA ingredients were isolated and prepared by TLC method, and their structure types were deduced by TLC chromogenic reaction, IR and 13C-NMR spectra. RESULTS The characters of chromogenic reaction, IR and 13C-NMR spectra of anti-MRSA ingredients produced by Streptomyces sp. ZZ027 and Streptomyces sp. ZZ021 were consistent with those of macrolides. CONCLUSION Anti-MRSA secondary metabolites produced by Streptomyces sp. ZZ027 and Streptomyces sp. ZZ021 were macrolides. |
| Key words: Streptomyces sp. ZZ027 Streptomyces sp. ZZ021 macrolides structure types identification |
