西他滨联合丙戊酸钠对白血病细胞株的促分化效应研究

Synergistic Effect of Decitabine and Valproic Acid on Induction of Differentiation in Leukemic HL-60 Cells

关键词:  分化抗原  HL-60细胞  丙戊酸钠  地西他滨

Key words:  differntiation antigen  HL-60  Valproic acid  Decitabine

摘要:

目的 探讨地西他滨(decitabine,DCA)和丙戊酸钠(valproic acid,VPA)联用对白血病细胞株HL-60的促分化影响。方法 设立分组如下：对照组，DCA A组(1.0 μmol·L^-1)，DCA B组(4.0 μmol·L^-1)，VPA组(2.0 mmol·L^-1)，联合用药A组(DCA 1.0 μmol·L^-1+VPA 2.0 mmol·L^-1)，联合用药B组(DCA 4.0 μmol·L^-1+VPA 2.0 mmol·L^-1)，作用48 h。流式细胞术检测CD34、CD117 MFI以及CD11b、CD14表达率。结果 联合用药A、B组的CD117和CD34 MFI显著低于其各自的单药组(P<0.01)；联合用药A组的CD11b和CD14表达率和联合用药B组CD11b表达率显著低于其各自的单药组(P<0.01)。结论 VPA与DCA联用对HL-60细胞具促分化作用。

Abstract:

OBJECTIVE To investigate the synergistic effect of decitabine(DCA) and valproic acid(VPA) in differentiation induction in leukemic HL-60 cells. METHODS The drug groups were set as follows: control group; DCA group A(1.0 μmol·L^-1); DCA group B(4.0 μmol·L^-1); VPA group(2.0 mmol·L^-1); combination group A(DCA 1.0 μmol·L^-1+VPA 2.0 mmol·L^-1); combination group B(DCA 4.0 μmol·L^-1+VPA 2.0 mmol·L^-1). The cells were treated for 48 h. The CD117, CD34 mean fluorescence intensity(MFI) and CD14, CD11b expression were detected by flow cytometry. RESULTS The CD117, CD34 MFI of combination group A and B were significantly lower than their corresponding concentration single drug group(P<0.01). Except CD14 of combination group B, the CD14, CD11b expressions of combination group A and B were significantly lower than their corresponding concentration single drug group(P<0.01). CONCLUSION There is an enhanced differentiation activity of combinations of DCA and VPA in HL-60 cells.