HPLC-ELSD测定丹参提取物中的单糖和二糖

Determination of Monosaccharide and Disaccharide in Tanshinon Extract by HPLC-ELSD

关键词:  丹参提取物  高效液相色谱-蒸发光散射  单糖  二糖

Key words:  Tanshinon extract  HPLC-ELSD  monosaccharide  disaccharide

摘要:

目的 建立一种以高效液相色谱-蒸发光散射(HPLC-ELSD)对丹参提取物中单糖和二糖进行测定的方法。方法 使用PrevailTM Carbohydrate ES色谱柱，以乙腈和水为流动相，梯度洗脱，柱温为35 ℃，流速为0.8 mL·min^-1；ELSD漂移管温度为60 ℃，载气为空气，气压为25 psi。结果 丹参提取物中单糖和二糖组分能够得到较好的分离，葡萄糖、果糖和蔗糖的线性范围分别为0.246~1.230，0.236~1.182，0.246~1.230 mg；平均加样回收率(n=6)分别为101.7%(RSD=1.6%)、102.0%(RSD=1.0%)和100.8%(RSD=0.9%)。结论 该方法快速简捷、结果准确稳定，适合于丹参提取物中单糖、双糖的含量测定。

Abstract:

OBJECTIVE An HPLC-ELSD method was established for the simultaneous quantitative analysis of monosaccharide, disaccharide from Tanshinon extract. METHODS The chromatographic separation was achieved on PrevailTM Carbohydrate ES column using a mobile phase composed of a mixture of acetonitrile and water by gradient elution at a flow rate of 0.8 mL·min^-1. The ELSD drift tube temperature was set at 60 ℃ and the carrying gas was air. RESULTS Monosaccharides and disaccharides from Tanshinon extract could be well separated, and the linear ranges of fructose, glucose and sucrose were 0.246-1.230 mg, 0.236-1.182 mg and 0.246-1.230 mg. The average spiked recoveries(n=6) of fructose, glucose and sucrose were 101.7%(RSD=1.6%), 102.0%(RSD=1.0%) and 100.8%(RSD=0.9%). CONCLUSION This method is accurate, rapid, simple and reproducible, and thus it is suitable for the determination of monosaccharides, disaccharides from Tanshinon extract.