新疆阿魏不同物候期阿魏酸含量动态变化研究

Study on Dynamic Changes of Ferulic Acid in Ferula Sinkiangensis in Different Phenological Period

关键词:  新疆阿魏  阿魏酸  高效液相色谱法  动态变化

Key words:  Ferula sinkiangensis  ferulic acid  HPLC  dynamic change

摘要:

目的 建立新疆阿魏中阿魏酸的含量测定方法，并对不同物候期新疆阿魏药材进行含量测定。方法 采用高效液相色谱法测定阿魏酸的含量，色谱柱为Symmetry Shield RP₁₈(3.9 mm×150 mm，5 μm)，流动相为乙腈-0.1%磷酸(20∶80)，检测波长：323 nm，流速：1 mL·min^-1，柱温：30 ℃。结果 阿魏酸线性范围为1.92~11.5 μg·mL^-1(r=0.999 8)，平均回收率为99.6%，RSD=1.50%(n=9)。不同物候期新疆阿魏中阿魏酸的含量在0.063~0.236 mg·g^-1，尤以两年生新疆阿魏中阿魏酸的含量较高。结论 本方法专属性强，重现性好，结果准确可靠，可作为阿魏的质量控制方法。

Abstract:

OBJECTIVE To establish a method for determination of the content of ferulic acid in Ferula sinkiangensis, and to determine the content of ferulic acid in Ferula sinkiangensis in different phenological period. METHODS The content of ferulic acid was determined by HPLC. The determination was performed on Symmetry Shield RP₁₈(3.9 mm×150 mm, 5 μm) column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solvent(20∶80). The UV wavelength was set at 323 nm. The flow rate was 1 mL·min^-1 and column temperature was 30 ℃. RESULTS The linear range of ferulic acid in Ferula sinkiangensis were 1.92-11.50 μg·mL^-1(r=0.999 8) with a mean recovery rate of 99.6％ and RSD was 1.50％(n=9). The content of ferulic acid in Ferula sinkiangensis of different years was within the range of 0.063-0.236 mg·g^-1. Especially the content of ferulic acid in Ferula sinkiangensis growed for two years was the highest. CONCLUSION The method is specific, reliable and accurate. It can be used for the quality control of Ferula sinkiangesis effectively.