银杏叶片中银杏黄酮在Beagle犬体内的药动学研究

Pharmacokinetics of Ginkgo Flavones in Ginkgo Leaf Tablets in Beagle Dogs

关键词:  银杏黄酮  槲皮素  高效液相色谱-电化学检测法  药动学

Key words:  ginkgo flavones  quercetin  HPLC-ECD  pharmacokinetics

摘要:

目的 以槲皮素为代表，研究银杏叶片中银杏黄酮类成分在Beagle犬体内的药动学特征。方法 Beagle犬口服给药银杏叶片后，按设定时间静脉采血。血样经固相萃取、酸水解后，以HPLC-ECD测定不同时间点血浆中槲皮素的浓度。应用DAS数据处理软件对数据进行统计学处理。结果 口服给药后，槲皮素在Beagle犬体内的药动学过程符合二房室模型，其主要药动学参数为t_1/2z 5.67 h，AUC_0-t 63.8 mg·L^-1·h，C_max 15.9 mg·L^-1。结论 建立的方法简单、灵敏、准确, 可用于研究以槲皮素为代表的银杏黄酮类成分的药动学特征，为银杏叶片的临床应用提供了依据。

Abstract:

OBJECTIVE To investigate pharmacokinetics of ginkgo flavones in Beagle dogs by quercetin, a typical aglycone of ginkgo flavones. METHODS Blood samples were collected at scheduled time points after a single oral administration of ginkgo leaf tablets. The concentration of quercetin in plasma was determined by HPLC-ECD after solid-phase extraction (SPE) and hydrolyzed by HCl. The main parameters of pharmacokinetics were calculated by DAS software. RESULTS The concentration-time course of quercetin was best fitted to a two-compartment model after oral administration. The main pharmacokinetic parameters were t_1/2z 5.67 h, AUC_0-t 63.8 mg·L^-1·h, C_max 15.9 mg·L^-1. CONCLUSION The method is simple, sensitive and accurate. It provides an important method for the determination and pharmacokinetics study of quercetin.