杏仁超临界CO2萃取物抗UVA对成纤维细胞的损伤作用

Protective Effects of Supercritical CO2 Extracts of Armeniacae Semen Amarum on Human Fibroblast Damaged by Ultraviolet A

关键词:  杏仁  超临界CO₂萃取  成纤维细胞  光老化

Key words:  Armeniacae Semen Amarum  supercritical CO₂ extracts  fibroblasts  photoaging

摘要:

目的 探讨杏仁超临界CO₂萃取物对UVA诱导的人皮肤成纤维细胞(ESF-1)损伤的保护作用及其作用机制。方法 用20 J·cm^-2的UVA照射ESF-1细胞建立光老化细胞模型，以不同浓度杏仁超临界CO₂萃取物处理光老化细胞，MTT法检测细胞活力，羟胺法检测细胞SOD活性，比色法检测GSH-Px活性，TBA法检测MDA含量，酶联免疫法检测TNF-α、IL-1β分泌量。结果 UVA照射ESF-1细胞后，细胞活力、SOD、GSH-Px活性降低，MDA含量升高，TNF-α、IL-1β分泌量增加(P<0.01)，40 μg·mL^-1和80 μg·mL^-1杏仁超临界CO₂萃取物能显著提高成纤维细胞活力、SOD、GSH-Px活性，降低MDA含量，减少TNF-α、IL-1β分泌(P<0.05或P<0.01)。结论 杏仁超临界CO2萃取物能减轻UVA对人皮肤成纤维细胞的损伤，其机制可能与其清除氧自由基，抗脂质过氧化，抑制氧化损伤，减少炎症细胞因子分泌有关。

Abstract:

OBJECTIVE To observe the protective of supercritical CO₂ extracts of Armeniacae Semen Amarum on human fibroblasts iniured by irradiation of ultraviolet A and explore its possible mechanism. METHODS The model of photoaging fibroblasts was established by irradiating of ultraviolet light(20 J·cm^-2). The different concentration of supercritical CO₂ extracts of Armeniacae Semen Amarum were used for photoaging cell. The cell viability, SOD activity, GSH-Px activity, MDA content were assayed by MTT, hydroxylamine, colorimetric and TBA methods, respectively. The secretion levels of TNF-α and IL-1β of cultured ESF-1 cells were detected by ELISA. RESULTS After the UVA irradiation, the cell viability, the activities of SOD and GSH-Px were decreased, the content of MDA, TNF-α, and IL-1β were increased(P<0.01). The above changes were inhibited(P<0.05 or P<0.01) by 40 μg·mL^-1 and 80 μg·mL^-1 supercritical CO₂ extracts of Armeniacae Semen Amarum. CONCLUSION The supercritical CO₂ extracts of Armeniacae Semen Amarum might inhibit the proliferation decreasing which induced by UVA. And its mechanism may have relationship with the ability of strengthening antioxidation, decreasing oxygen radicals and decreasing the secretions of proinflammatory cytokines.