硒化玉米须多糖的工艺条件及硒含量测定研究

Study on the Synthesis Processing and Content Determination of Corn Silk Polysaccharide Selenide

关键词:  玉米须多糖  亚硒酸钠  硒化  正交试验

Key words:  corn silk polysaccharide  sodium selenite  selenizing  orthogonal test

摘要:

目的 以玉米须多糖为原料，用亚硒酸钠进行玉米须多糖的硒化研究。方法 利用单因素和正交试验确立硒化的最佳工艺条件；利用硒-硫氰酸钾-甲基紫萃取光度法测定硒多糖中的硒含量，并通过红外光谱对硒多糖进行了初步表征。结果 最佳工艺条件为反应温度70 ℃，反应时间8 h，玉米须多糖与亚硒酸钠质量比为1∶1.2，硝酸体积分数为0.3%，玉米须硒多糖中硒含量为3.17 mg·g^-1，平均收率为35.72%。红外光谱显示：玉米须硒多糖中含有Se=O键和Se-C键。结论 利用该工艺成功合成了玉米须硒多糖，为玉米须的开发和利用奠定基础。

Abstract:

OBJECTIVE Using the polysaccharide from corn silk as the raw material, to study the selenizing process with sodium selenite. METHODS Selenizing conditions were established by single factor and orthogonal design; determining content of polysaccharide selenide by Se(Ⅳ)-KSCN-MV extraction spectrophotometry; characterizing the structure of polysaccharide selenide by IR. RESULTS The optimal conditions were: 70 ℃ reaction temperature, 8 hours reaction time, mass ratio of corn silk polysaccharide to sodium selenite 1∶1.2, 0.3% HNO3 of volume fraction. The average content of selenium in corn silk polysaccharide was over 3.17 mg·g^-1 and average yield was 35.72%. IR results showed that corn silk polysaccharide contained Se=O and Se-C. CONCLUSION Corn silk polysaccharide selenide is successfully synthesized and the study provides the foundation for further study and exploiting for corn silk.