HPLC同时测定复方替硝唑载体栓中3组分含量

Simultaneous Determination of Three Components in Compound Tinidazole Carrier Suppositories by HPLC

关键词:  复方替硝唑载体栓  替硝唑  克霉唑  醋酸氯己定  高效液相色谱法

Key words:  compound tinidazole carrier suppositories  tinidazole  clotrimazole  chlorhexidine acetate  HPLC

摘要:

目的 建立HPLC同时测定复方替硝唑载体栓中3组分含量。方法 采用Diamonsil C₁₈(200 mm×4.6 mm，5 μm)；流动相：甲醇-水-三乙胺(60∶40∶0.5)(用磷酸调pH值至4.0)，流速：1.0 mL·min^-1，检测波长：260 nm，进样量：20 μL；柱温：25 ℃。结果 复方替硝唑载体栓中3组分替硝唑、克霉唑、醋酸氯己定的线性范围分别为40~600，32~480，1.6~24 μg·mL^-1(r=0.999 9)，平均回收率分别为99.81％，100.2％，100.3％；RSD分别为0.37％，0.2％和0.4％(n=9)。结论 该方法可同时测定3种成分含量，操作简便、快速，结果准确、可靠。

Abstract:

OBJECTIVE To establish an HPLC method to simultaneous determine three components in compound tinidazole carrier suppositories. METHODS A Diamonsil-C₁₈(200 mm×4.6 mm, 5 μm) column was used; the mobile phase was the mixture of methanol-water-triethylamine(60∶40∶0.5)(adjusted by phosphoric acid to pH 4.0), the flow rate was 1.0 mL·min^-1, the UV detector was operated at 260 nm, injection volume was 20 μL; column temperature was 25 ℃. RESULTS The linear ranges of three components in compound tinidazole carrier suppositories tinidazole, clotrimazole, chlorhexidine acetate were 40–600, 32–480, 1.6–24 μg·mL^-1(r=0.999 9). The average recoveries respectively were 99.81%, 100.2%, 100.3%, and RSDs respectively were 0.37%, 0.2% and 0.4%(n=9). CONCLUSION This method can simultaneously determine three components. It is easy and fast with accurate and reliable results.