| 引用本文: | 周浓,杨勤,李洁玉,施晓燕,姜北.川楝子乙醇部位HPLC指纹图谱研究[J].中国现代应用药学,2012,29(9):814-818. |
| ZHOU Nong,YANG Qin,LI Jieyu,SHI Xiaoyan,JIANG Bei.HPLC Fingerprints of Ethanolic Part of Toosendan Fructus[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(9):814-818. |
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| 摘要: |
| 目的 建立川楝子乙醇提取部位的HPLC指纹图谱。方法 色谱柱Agilent zorbax SB-C18柱(4.6 mm×150 mm，5 μm)；流动相甲醇/乙腈(1∶10)(A)-0.4%磷酸溶液(B)，梯度洗脱；检测波长360 nm；柱温25 ℃；流速1.0 mL·min-1。结果 建立了川楝子药材乙醇提取部位的HPLC色谱指纹图谱。在指纹图谱中共有峰20个，并指认了3个色谱峰。结论 该方法准确可靠，重复性好，可为川楝子药材及其饮片的质量控制提供参考依据。 |
| 关键词: 川楝子 乙醇部位 高效液相色谱法 指纹图谱 |
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| HPLC Fingerprints of Ethanolic Part of Toosendan Fructus |
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ZHOU Nong1,2, YANG Qin3, LI Jieyu3, SHI Xiaoyan4, JIANG Bei4
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1.College of Pharmacy, Dali University, Dali 671000, China;2.College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, China;3.Chongqing Three Gorges Medical College, Chongqing 404020, China;4.College of Pharmacy, Dali University, Dali 671000, China
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| Abstract: |
| OBJECTIVE To establish HPLC fingerprints of ethanolic extraction part of Toosendan Fructus. METHODS Agilent zorbax SB-C18 column (4.6 mm×150 mm, 5 μm) was adopted. A gradient elution was applied with mobile phase A (methanol and acetonitrile 1∶10) and mobile phase B (0.4% phosphoric acid solution). The flow rate was 1.0 mL·min-1, the detective wavelength was 360 nm, and the column temperature was 25 ℃. RESULTS HPLC fingerprints of ethanolic extraction part of Toosendan Fructus was established. Twenty peaks were observed on the HPLC fingerprints of Toosendan Fructus and 3 of them were identified. CONCLUSION The method developed in this study is accurate with high repeatability, and available for the quality control of Toosendan Fructus and its decoction pieces. |
| Key words: Toosendan Fructus ethanolic part HPLC fingerprint |
