HPLC测定野马追中野马追内酯A，B和金丝桃苷的含量

Determination of Eupalinolide A, B and Hyperin in Eupatorium Lindleyanum DC by HPLC

关键词:  野马追  野马追内酯A  野马追内酯B  金丝桃苷  高效液相色谱法

Key words:  Eupatorium lindleanum DC.  eupalinolide A  eupalinolide B  hyperin  HPLC

摘要:

目的 采用高效液相色谱法对不同产地的6批野马追药材中野马追内酯A，B和金丝桃苷进行了定性定量分析。方法 野马追内酯A，B的测定采用Agilent Extend-C₁₈(4.6 mm×150 mm，5 μm)为分析柱，乙腈-水(27∶73)为流动相，流速为1.0 mL·min^-1，等度洗脱，检测波长为220 nm，柱温为30 ℃。金丝桃苷的测定以SB-C₁₈为分析柱，乙腈-1%醋酸水溶液(12∶88)为流动相，流速为1.0 mL·min^-1，等度洗脱，检测波长为255 nm。结果 野马追内酯A在0.02~1.88 μg，野马追内酯B在0.44~4.44 μg内与峰面积呈良好的线性关系。金丝桃苷在0.101~1.414 μg内与峰面积呈良好的线性关系。结论 本实验方法准确、快速、灵敏、重复性好，在该色谱条件及样品处理方法下，可以准确测定野马追中野马追内酯A，B和金丝桃苷的含量。

Abstract:

OBJECTIVE To establish a method for the determination of the content of eupalinolide A, B and hyperin in Eupatorium lindleyanum DC from 6 different areas by HPLC. METHODS For eupalinolide A, B, Agilent Extend-C₁₈ column (4.6 mm×150 mm, 5 μm) was used under the mobile phase of acetonitrile-water(27∶73) at the flow rate of 1.0 mL·min^-1. The detection wavelength was 220 nm and the column temperature was maintained at 30 ℃. For hyperin, SB-C₁₈ column was used under the mobile phase of acetonitrile-1% Acetic acid(12∶88) at a flow of 1.0 mL·min^-1. The detection wavelength was 255 nm and the column temperature was maintained at 30 ℃. RESULTS The standard curve of eupalinolide A was linear in the range of 0.02-1.88 μg and eupalinolide B was linear in the range of 0.44-4.44 μg. The standard curve of hyperin was linear in the range of 0.101-1.414 μg. CONCLUSION This method was simple, accurate and reproducible for the determination of eupalinolide A, B and hyperin in Eupatorium lindleyanum DC.