| 引用本文: | 王毓菁，张根元，王德才，陶伟博，戈文兰.HPLC测定片剂中阿戈美拉汀的含量[J].中国现代应用药学,2012,29(7):652-655. |
| WANG Yujing, ZHANG Genyuan, WANG Decai, TAO Weibo, GE Wenlan.Determination of Agomelatine in Tablets by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(7):652-655. |
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| 摘要: |
| 目的 采用HPLC测定片剂中阿戈美拉汀的含量。方法 色谱柱为Phenomenex Luna-C18(250 mm×4.6 mm，5 μm)，流动相为甲醇-0.05 mol·L-1磷酸二氢钠溶液(40∶60)，流速为1.0 mL·min-1，检测波长为230 nm, 柱温为25 ℃。结果 线性回归方程为Y=1.979×105X-1.258×104(r=0.999 9，n=5)，线性范围为1.07~10.7 μg·mL-1，平均回收率为98.73% (n=9)，RSD为1.28%。结论 所建方法简便、准确、专属，可用于片剂中阿戈美拉汀的含量测定。 |
| 关键词: 阿戈美拉汀 高效液相色谱法 含量测定 |
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| Determination of Agomelatine in Tablets by HPLC |
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WANG Yujing, ZHANG Genyuan, WANG Decai, TAO Weibo, GE Wenlan1,2
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1.Nanjing University of Technology, Nanjing 211816, China;2.Nanjing University of Technology Jiangsu Provincial Institute of Material Medical, Nanjing 210009, China
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| Abstract: |
| OBJECTIVE To establish a method for the content determination of Agomelatine tablets by HPLC. METHODS The HPLC method was performed on Phenomenex Luna-C18 column (250 mm×4.6 mm, 5 μm) with mobile phase of methanol-0.05 mol·L-1 sodium dihydrogen phosphate (40∶60). Flow rate was 1.0 mL·min-1. Detective wavelength was set at 230 nm and the column temperature was set at 25 ℃. RESULTS The calibration curve of Agomelatine was linear Y=1.979×105X-1.258×104 (r=0.999 9, n=5) in the range of 1.07-10.7 μg·mL-1. The average recovery of Agomelatine was 98.73% (n=9) with RSD of 1.28%. CONCLUSION The method is simple, accurate, specific and it can be used for the determination of the content of Agomelatine tablets. |
| Key words: Agomelatine HPLC content determination |
