| 引用本文: | 冯素香,吴加,李建生,屈凌波,徐会平,杨冉,李建军.大黄5个蒽醌类成分与大鼠血浆蛋白结合率的测定[J].中国现代应用药学,2013,30(1):1-5. |
| FENG Suxiang,WU Jia,LI Jiansheng,QU Lingbo,XU Huiping,YANG Ran,LI Jianjun.Determination of the Binding Rate of Rat Plasma Protein with Five Anthraquinones in Rhubarb[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(1):1-5. |
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| 摘要: |
| 目的 建立测定大黄苷元中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚5种成分与大鼠血浆蛋白结合率的方法。方法 采用体外平衡透析法，模拟大黄苷元与血浆蛋白的结合过程，以固相萃取柱处理血浆样品，用高效液相色谱法测定大黄苷元中5种蒽醌类成分在透析袋内血浆中的浓度与透析袋外缓冲液中的浓度，计算血浆蛋白结合率。结果 芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚提取回收率分别为68.6%，91.5%，78.6%，88.2%和71.4%，与大鼠体外血浆蛋白结合率分别为93.1%，91.3%，95.2%，87.5%和89.7%。结论 大黄5种苷元与大鼠血浆蛋白结合率较高，且蛋白结合率与血药浓度无明显依赖性。 |
| 关键词: 大黄苷元 血浆蛋白结合率 平衡透析法 高效液相色谱法 |
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| 基金项目:国家自然科学基金资助项目(81274179)；河南省高校青年骨干教师资助项目(2009GGJS-065)；河南省教育自然科学研究计划项目(2010A360002) |
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| Determination of the Binding Rate of Rat Plasma Protein with Five Anthraquinones in Rhubarb |
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FENG Suxiang1,2, WU Jia1, LI Jiansheng1, QU Lingbo2,3, XU Huiping2, YANG Ran2, LI Jianjun2
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1.Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;2.Zhengzhou University, Zhengzhou 450001, China;3.Henan University of Technology, Zhengzhou 450001, China
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| Abstract: |
| OBJECTIVE To develop a method for the simultaneous determination of the binding rate of plasma protein with aloe-emodin, rhein, emodin, chrysophanol and physcion. METHODS The equilibrium dialysis method was carried out to imitate the binding process between five anthraquinones and plasma protein. Plasma samples were treated with solid-phase extraction and the binding rate was determined by HPLC. RESULTS The extract recoveries of aloe-emodin, rhein, emodin, chrysophanol and physcion were 68.6%, 91.5%, 78.6%, 88.2% and 71.4%, the binding rates of plasma protein were 93.1%, 91.3%, 95.2%, 87.5% and 89.7%. CONCLUSION This method shows the high binding rate of five anthraquinones to plasma protein. The binding rates of five anthraquinones are not proportionally dependent on plasma concentration. |
| Key words: rhubarb aglycone protein binding rate equilibrium dialysis HPLC |
