转运体稳定表达的验证方法及其研究进展

Methods and Progress in the Study of Validating the Stable Expression of Drug Transporters

关键词:  转运体  转录水平  蛋白表达  活性验证

Key words:  transporters  transcription level  protein expression  activity assessment

摘要:

目的 介绍验证转运体稳定表达的方法和技术，为转运体细胞模型的应用提供依据。方法 对近年来研究转运体在细胞模型中稳定表达的相关文献进行综述。结果 实时定量PCR及Western blot是分别验证转运体转录水平和蛋白表达的经典方法。结论 与荧光检测技术相结合的验证方法在转录水平、蛋白表达与功能活性等三方面都显示出广阔的应用前景。

Abstract:

OBJECTIVE To introduce approaches and techonologies for validating the stable expression of drug transporters in vitro, and provide suggestions for application in cellular models. METHODS Review related literatures about the recent research progress on stably expressing transporters in transfected cells. RESULTS Real-time polymerase chain reaction and Western blot were classical methods respectively for detecting the expression of messenager ribonucleic acid (mRNA) and protein. CONCLUSION The combination of real time-PCR,Western blot with fluorescence detection techonology have promising applications in verifying the stable expression of transporter in different levels including transcription, protein expression and functional activity.