| 引用本文: | 窦志华,罗琳,候金燕,张琳,杨爱华,安莉萍.茵陈蒿汤保肝作用的血清药理学研究[J].中国现代应用药学,2012,29(10):868-871. |
| DOU Zhihua,LUO Lin,HOU Jinyan,ZHANG Lin,YANG Aihua,AN Liping.Studies on Liver Protection of Yinchenhao Decoction by Seropharmacological Method[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(10):868-871. |
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| 摘要: |
| 目的 建立茵陈蒿汤血清药理学研究方法。方法 建立CCl4诱导的大鼠肝细胞损伤细胞模型，SD大鼠为含药血清供体，以含药血清对损伤肝细胞增殖(WST-8法)的影响为指标，采用正交试验法优选含药血清的制备方法，并确定体系中血清添加量。结果 每天以成人等效剂量10倍的茵陈蒿汤水提醇沉液间隔8 h分2次给正常大鼠灌胃给药，连续3 d，末次给药后1 h采集血浆，体系中加入20%含药血清能明显提高损伤肝细胞的增殖能力。结论 适宜条件下制备的茵陈蒿汤含药血清对CCl4诱导的肝细胞损伤具有明显保护作用，血清药理学研究方法可以用于该方剂的药效评价。 |
| 关键词: 茵陈蒿汤 保肝作用 含药血清 血清药理学 |
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| 基金项目:江苏省高校自然科学基础研究项目(08KJB360009)；江苏省中医药局科研计划项目(HZ07071) |
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| Studies on Liver Protection of Yinchenhao Decoction by Seropharmacological Method |
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DOU Zhihua1, LUO Lin2, HOU Jinyan3, ZHANG Lin1, YANG Aihua3, AN Liping3
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1.Third Affiliated Hospital of Nantong University, Nantong 226006, China;2.Nantong University, Nantong 226001, China;3.Nanjing University of Chinese Medicine, Nanjing 210046, China
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| Abstract: |
| OBJECTIVE To establish a seropharmacological method for Yinchenhao decoction. METHODS Model of CCl4-induced injury of hepatocyte was established and rats were used as the donor to prepare Yinchenhao decoction containing serum. Hepatocyte proliferation and orthogonal experiment was carried out to screen the preparative method of drug-containing serum and the suitable concentration in culture system. RESULTS Normal rats as the donator of serum, ten times equivalent dose of the extract of Yinchenhao decoction for adults was used. The optimal preparative method of drug-contained serum was orally administration twice a day for 3 days, plasma was collected after 1 h of the last administration. Addition of 20% drug-contained serum to culture system could elevate hepatocyte proliferation significantly. CONCLUSION Yinchenhao decoction containing serum prepared by suitable method possesses an obvious protective effect on CCl4-induced hepatocyte injury and seropharmacology can be applied to evaluate the pharmacological effect of Yinchenhao decoction. |
| Key words: Yinchenhao decoction liver protection drug-containing serum seropharmacology |
