HPLC同时测定乳膏中尿囊素和烟酰胺的含量

Simultaneous Determination of Allantoin and Nicotinamide in Cream by HPLC

关键词:  尿囊素  烟酰胺  高效液相色谱法  含量测定

Key words:  allantoin  nicotinamide  HPLC  content determinntion

摘要:

目的 建立以高效液相色谱法同时测定乳膏中尿囊素和烟酰胺含量的方法。方法 采用Zorbax SB C₁₈色谱柱(4.6 mm×250 mm，5 μm)，流动相：甲醇-0.02 mol·L^-1磷酸二氢钾溶液(25∶75)，流速：0.5 mL·min^-1，柱温：30 ℃，检测波长：217 nm。结果 尿囊素在4.2~21.0 μg·L^-1内线性关系良好(r=0.999 9)，烟酰胺在8.0~40.0 μg·L^-1内线性关系良好(r=0.999 7)；尿囊素和烟酰胺平均加样回收率分别为99.4%(RSD=0.27%)和99.7%(RSD=0.25%)。结论 该方法简便、快速、准确，可用于制剂的质量控制。

Abstract:

OBJECTIVE To establish an HPLC method for determination of allantoin and nicotinamide in cream. METHODS Zorbax SB-C₁₈ column(250 mm×4.6 mm, 5 μm) was used as stanationly phase with methanol-0.02 mol·L^-1 potassium dihydrogen phosphate(25∶75) as mobile phase. The flow rate was 0.5 mL·min^-1, the column temperature was 30 ℃, and the detection wavelength was set at 217 nm. RESULTS The method had good linear relationship within the range of 4.2-21.0 μg·L^-1(r=0.999 9) for allantoin and 8.0-40.0 μg·L^-1(r=0.999 7) for nicotinamide, respectively. The results of the average recovery were 99.4%(RSD=0.27%) and 99.7%(RSD=0.25%). CONCLUSION The method is simple, rapid and accurate, and it can be used for the quality control.