HPLC同时测定大血藤中红景天苷和绿原酸的含量

Simultaneous Determination of Salidroside and Chlorogenic Acid in Sargentodoxa Cueata by HPLC

关键词:  大血藤  红景天苷  绿原酸  波长转换法

Key words:  Sargentodoxa cueata  salidroside  chlorogenic acid  wavelength conversion method

摘要:

目的 建立高效液相转换波长法，同时测定大血藤中红景天苷和绿原酸的含量。方法 采用Inertsil ODS-SP色谱柱(4.6 mm×250 mm，5 μm)，乙腈-0.1%磷酸水溶液梯度洗脱；流速1 mL·min^-1，柱温30 ℃，检测波长220，325 nm。结果 红景天苷和绿原酸分别在0.061 7~1.234 μg(r=0.999 7)和0.030 2~0.605 μg(r=0.999 9)与峰面积呈良好的线性关系，平均回收率(n=6)分别为97.8%和98.1%，RSD分别为1.9%和1.7%。结论 该方法同时测定大血藤中的红景天苷和绿原酸的含量，方法简单、准确度高、重复性好，可为大血藤药材的质量控制提供参考。

Abstract:

OBJECTIVE To establish a wavelength conversion method for determination of salidroside and chlorogenic acid in Sargentodoxa cueata. METHODS The separation was performed on a Inertsil ODS-SP column(4.6 mm×250 mm, 5 μm). Acetonitril-0.1% H₃PO₄ was used as the mobile phase with gradient elution. The flow rate was 1 mL·min^-1. The detection wavelength was 220 nm and 325 nm. RESULTS The linear range was 0.061 7-1.234 μg(r=0.999 7) and 0.030 2-0.605 μg (r=0.999 9), respectively. The average recoveries(n=6) of salidroside and chlorogenic acid were 97.8% and 98.1%, respectively. CONCLUSION This method is simple, sensitive and it may be used for the quality control of Sargentodoxa cuneata.