UPLC同时测定不同产地川芎中8种活性成分的含量

Simultaneous Determination of Eight Active Components in Chuanxiong Rhizoma from Different Areas by UPLC

关键词:  川芎  活性成分  UPLC

Key words:  Chuanxiong Rhizoma  active components  UPLC

摘要:

目的 建立同时测定不同产地川芎中咖啡酸、香草醛、阿魏酸、洋川芎内酯I、洋川芎内酯H、丁基苯酞、藁本内酯和丁烯基苯酞含量的UPLC方法。方法 采用ACQUITY UPLC，HSS T3色谱柱(2.1 mm×100 mm，1.8 μm)，流动相为甲醇-0.03%磷酸水溶液，梯度洗脱，PDA检测器，针对不同组分，选择其最大吸收波长作为检测波长。结果 咖啡酸、香草醛、阿魏酸、洋川芎内酯I、洋川芎内酯H、丁基苯酞、藁本内酯和丁烯基苯酞分别在0.082 8~3.312 μg·mL^-1，0.047 85~ 1.914 μg·mL^-1，4.420~176.8 μg·mL^-1，2.035~81.4 μg·mL^-1，0.470 4~18.82 μg·mL^-1，1.044~41.75 μg·mL^-1，13.55~542.0 μg·mL^-1，1.785~71.40 μg·mL^-1内呈良好的线性关系，平均回收率分别为101.0%，99.1%，98.9%，103.6%，96.3%，102.8%，98.1%，98.0%。结论 该方法简便、快捷、重复性好，可用于川芎药材及其制剂的质量控制。

Abstract:

OBJECTIVE To develop a UPLC method for determination of caffeic acid, vanillin, ferulic acid, senkyunolide I, senkyunolide H, butylphthalide, ligustilide and butylidenephthalide in Chuanxiong Rhizoma from different areas. METHODS The analysis was carried out on an HSS T3 (2.1 mm×100 mm, 1.8 μm) column. The mobile phase was composed of 0.03% phosphoric acid aqueous and methanol with gradient elution. The detection wavelengths were set at three different wavelengths according to different components. RESULTS The standard curves of eight active components showed a good linearity in 0.082 8-3.312 μg·mL^-1, 0.047 85-1.914 μg·mL^-1, 4.420-176.8 μg·mL^-1, 2.035-81.4 μg·mL^-1, 0.470 4-18.82 μg·mL^-1, 1.044-41.75 μg·mL^-1, 13.55-542.0 μg·mL^-1, 1.785-71.40 μg·mL^-1. The average recoveries were 101.0%, 99.1%, 98.9%, 103.6%, 96.3%, 102.8%, 98.1%, 98.0%, respectively. CONCLUSION This method is simple, quick, reproducible and can be used to control the quality of Chuanxiong Rhizoma and its preparations.