香椿子总多酚对佐剂型关节炎大鼠的治疗作用

Effects of Total Polyphenols from Seeds of Toona Sinensis in Treating Adjuvant-induced Arthritis Rats

关键词:  香椿子多酚  佐剂型关节炎  细胞因子  脏器指数  足跖肿胀度

Key words:  total polyphenols from seeds of Toona sinensisey  adjuvant-induced arthritisey  cytokines  visceral organs index  pedal swelling

摘要:

目的 研究香椿子总多酚(TSTP)对佐剂型关节炎大鼠的治疗作用。方法 6周龄Wistar大鼠50只，SPF级，随机分为5组，每组10只：正常组、模型组、雷公藤甲素组、TSTP低剂量组和TSTP高剂量组。正常组在左后肢足垫部皮下注射0.1 mL生理盐水，其余组在左后肢足垫部皮下注射0.1 mL弗氏完全佐剂(Freund's complete adjuvant，FCA)诱导建立大鼠佐剂型关节炎模型。大鼠致炎后第8天开始灌胃治疗，正常组和模型组均给予0.5%羧甲基纤维素钠(10 mg·kg^-1)、雷公藤甲素组给予雷公藤甲素(20 mg·kg^-1)、TSTP低剂量组给予TSTP(35 mg·kg^-1)、TSTP高剂量组给予TSTP(70 mg·kg^-1)，1次·d^-1，连续灌胃21 d。大鼠于致炎前(d0)、致炎后第4，8，12，16，20，24，28天(d4，d8，d12，d16，d20，d24，d28)分别在足容积测量仪上测定右侧(非致炎侧)足容积，d4，d8，d12，d16，d20，d24，d28与d0足容积的差值即为该时大鼠足趾肿胀度。最后一次灌胃结束2 h后，处理大鼠，同时摘取胸腺和脾脏组织并称重，计算胸腺指数和脾脏指数。并于踝关节上方0.5 cm剪取踝关节，采用苏木精-伊红染色法制作病理组织切片，观察形态学的相关改变。结果 与模型组比较，TSTP降低了脏器指数、足跖肿胀度的水平，差异有统计学意义(P<0.05)。与雷公藤组相比，TSTP高剂量组脏器指数、足跖肿胀度无明显差别；与TSTP高剂量组相比，TSTP低剂量组脏器指数、足跖肿胀度较高(P<0.05)。结论 TSTP高，低剂量各组均能够减轻佐剂关节炎大鼠的足趾肿胀度、降低脾脏指数和胸腺指数，改善踝关节病理组织形态。TSTP对佐剂型关节炎大鼠具有一定的治疗作用。

Abstract:

OBJECTIVE To research the effects of total polyphenols from seeds of Toona sinensis(TSTP) in treating Wistar rats suffering from adjuvant arthritis. METHODS The Wistar rats were randomized into normal group, model group, triptolide(20 mg·kg^-1) group, low-dose TSTP group(35 mg·kg^-1) and high-dose TSTP group(70 mg·kg^-1). Adjuvant-induced arthritis models were induced by subcutaneous injection of Freund's complete adjuvant on the left fore paw in rats. On the 8th day after immunization, rats had been treated with gastric infusion until the 21st day, once a day. The rats in normal and model group were treated with 0.5% sodium carboxymethylcell-ulose(10 mg·kg^-1). the Triptolide group were treated with triptolide (20?mg·kg^-1). The low-dose TSTP group was treated with TSTP 35 mg·kg-1. The high-dose TSTP group were treated with TSTP 70 mg·kg^-1. After 2 h of the last gastric infusion, the effects of treatment were valuated by measurement of the swelling degree in jionts, thymus index and spleen index. To observe the morphology changes, ankle joints were cut down and prepared for the tissue slice after hematoxylineosin staining. RESULTS Compared with model group, TSTP decreased the level of visceral organs index, pedal swelling. The difference was significant(P<0.05). Compared with triptolide group, there was no significant difference in high-dose TSTP group(P>0.05) in organs index and pedal swelling, and it was significant between high-doses TSTP group and low-dose TSTP group(P<0.05). CONCLUSION TSTP can reduce the swelling degree, thymus index and spleen index, and improve the pathomorphological changes of ankle joints in Wistar rats suffering from adjuvant arthritis, it indicates that TSTP has certain effects on adjuvant-induced arthritis rats.