| 引用本文: | 刘金华,郭倩倩,华海婴,袁波,赵永星,黄永焯.穿膜肽提高纳米银穿膜活性的研究[J].中国现代应用药学,2012,29(10):865-868. |
| LIU Jinhua,GUO Qianqian,HUA Haiying,YUAN Bo,ZHAO Yongxing,HUANG Yongzhuo.The Penetrating Activity of Nano-silver Mediated by Cell Penetrating Peptide[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(10):865-868. |
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| 摘要: |
| 目的 合成纳米银，在其表面修饰穿膜肽(TAT)，并检测修饰后纳米银粒对人乳腺癌耐阿霉素细胞(MCF-7/ADR)的穿膜活性。方法 通过化学还原法制备纳米银(AgNP)，并通过Ag-S共价键与TAT连接修饰AgNP。通过粒度仪、透射电镜、激光共聚焦显微镜、流式细胞仪以及二喹林甲酸法等仪器及方法对其表征、共价连接及TAT的介导活性进行测定。结果 成功制备了10 nm以下的AgNP，且修饰TAT后的AgNP(AgNP-TAT)表现出了比AgNP 更强的穿膜活性。结论 TAT修饰AgNP后能显著提高其穿膜活性。 |
| 关键词: 纳米银 穿膜肽 MCF-7/ADR 细胞摄取 |
| DOI： |
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| 基金项目:国家自然科学基金(91029743；81172996) |
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| The Penetrating Activity of Nano-silver Mediated by Cell Penetrating Peptide |
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LIU Jinhua1,2, GUO Qianqian1,2, HUA Haiying3, YUAN Bo4, ZHAO Yongxing3, HUANG Yongzhuo5
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1.Zhengzhou University, Zhengzhou 450001, China;2.Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai 201203, China;3.Zhengzhou University, Zhengzhou 450001, China;4.Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Ji’nan 250000, China;5.Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai 201203, China
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| Abstract: |
| OBJECTIVE To assess the intracellular delivery efficiency of silver nanoparticles surface-modified by cell penetrating peptides(TAT) in human breast cancer drug doxorubicin-resistant MCF-7/ADR cells. METHODS Silver nanoparticle(AgNP) was prepared by chemical reduction and was modified with TAT by Ag-S covalent bond. The TAT-AgNP was characterized using particle analyzer, TEM, laser confocal microsopy, and TGA, and TAT-mediated cellular uptake were measured using FACS. RESULTS The AgNP with diameter below 10 nm was successfully synthesized. The cell penetrating activity of TAT-modified AgNP (AgNP-TAT) was higher than that of AgNP. CONCLUSION The cell penetrating activity of AgNP was significantly improved by modification with TAT. |
| Key words: silver nanoparticles cell penetrating peptide MCF-7/ADR cell uptake |
