| 引用本文: | 石云峰,李会林,林丽琴.HPLC测定炔雌醚含量和有关物质[J].中国现代应用药学,2013,30(1):95-98. |
| SHI Yunfeng,LI Huilin,LIN Liqin.Determination of Quinestrol and Its Related Substances by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(1):95-98. |
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| 摘要: |
| 目的 建立HPLC测定炔雌醚含量和有关物质的方法。方法 采用C18柱，以甲醇-水(90∶10)为流动相；流速为1.0 mL·min-1；检测波长为279 nm。结果 炔雌醚的线性范围为0.078 8~0.788 4 mg·mL-1(r=1.000 0)，平均回收率为99.9%，RSD为0.80%(n=9)，炔雌醚检测限浓度为0.06 mg·mL-1。结论 该方法简便、准确、专属性强，可作为产品质量检测方法。 |
| 关键词: 炔雌醚 高效液相色谱法 有关物质 |
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| Determination of Quinestrol and Its Related Substances by HPLC |
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SHI Yunfeng1, LI Huilin2, LIN Liqin2
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1.Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China;2.Hangzhou Institute for Drug Control, Hangzhou 310017, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for content determination of quinestrol and its related substances. METHODS A C18 column was used with the mobile phase of water-methanol(90∶10). The flow rate was 1.0 mL·min-1, and the detection wavelength was 279 nm. RESULTS The calibration curve of quinestrol was linear in the range of 0.078 8-0.788 4 mg·mL-1(r=1.000 0), and the average recovery was 99.9%, RSD was 0.80%(n=9). The detection limit of quinestrol was 0.06 μg·mL-1. CONCLUSION The method is rapid, accurate and reliable for content determination of quinestrol and related substances. |
| Key words: quinestrol HPLC related substance |
