板蓝根活性组分预防肥胖作用及机制研究

Effect and Mechanism of Active Component of Isatidis Radix in Obesity Prevention

关键词:  肥胖  板蓝根活性组分  3T3-L1前脂肪细胞  预防肥胖

Key words:  obesity  active component of Isatidis Radix  3T3-L1 preadipocytes  obesity prevention

摘要:

目的 探讨板蓝根活性组分(ACIR)的预防肥胖作用及其可能的作用机制。方法 高脂饮食诱导小鼠肥胖的同时给予不同剂量ACIR，观察小鼠体质量、进食量、脂肪重量、脂肪细胞形态、肝脏重量及血脂变化，并进行小鼠负重游泳实验；体外培养诱导分化3T3-L1前脂肪细胞，观察ACIR对脂肪细胞增殖、分化和脂滴形成的影响。结果 ACIR能显著降低小鼠体质量、脂肪系数及肝脏重量，使脂肪细胞变小；改善小鼠进食量，明显延长小鼠负重游泳时间，使血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)含量降低，但高密度脂蛋白(HDL)无明显变化；抑制前脂肪细胞增殖和分化，减少脂滴形成。结论 ACIR具有预防肥胖和降血脂作用，其可能的机制是抑制前脂肪细胞增殖和分化。

Abstract:

OBJECTIVE To investigate the preventing obesity effect of active component of Isatidis Radix (ACIR) and its mechanism. METHODS Body weight, food intake, fat weight, fat cell morphology, liver weight and lipids of obese mice induced by a high-fat diet were chosen as the indexes to observe the preventing obesity effect of different dosages of ACIR. The swimming time of loaded mice under the action of drugs was also observed. 3T3-L1 preadipocytes were cultured to investigate the effect of ACIR on proliferation, differentiation and lipid droplet formation of 3T3-L1 preadipocytes. RESULTS ACIR could significantly decrease the body weight, fat index and the weight of the liver, at the same time reduce lipocyte size, improve food intake in mice, extend the swimming time of mice and serum total cholesterol (TC), triglycerides (TG), low density lipoprotein decreased (LDL), but high density lipoprotein (HDL) did not change; it could also inhibit the proliferation, differentiation and lipid droplet formation of 3T3-L1 preadipocytes. CONCLUSION ACIR has protective effect on preventing obesity, and can reduce blood lipids, which might be related to inhibiting the proliferation and differentiation of preadipocytes.