HPLC-DAD测定虎杖中八种成分含量及指纹图谱研究

Content Determination of Eight Components and Fingerprint Chromatograms of Polygoni Cuspidati Rhizoma Et Radix by HPLC-DAD

关键词:  虎杖  指纹图谱  高效液相色谱法  二极管阵列检测器

Key words:  Polygoni Cuspidati Rhizoma et Radix  fingerprint chromatogram  HPLC  DAD

摘要:

目的 利用HPLC-DAD对不同产地的虎杖药材进行分析，建立药材鉴别和质量控制方法。方法 采用YMC C₁₈(4.6 mm×250 mm，5 μm)柱，流动相为乙腈-0.2%磷酸水；梯度洗脱，检测波长为280 nm；流速为1.0 mL·min^-1；柱温为25 ℃。对5批虎杖药材进行分析，测定了8种成分的含量，并建立虎杖药材的指纹图谱。结果 8种成分的含量测定方法有良好的精密度、重现性、稳定性。建立的指纹图谱各批次药材均有17个共有峰，相似度范围为0.915~0.973。结论 综合指标成分定量测定及化学指纹图谱分析方法能较全面地表征不同产地虎杖的内在成分差异，可为虎杖质量控制提供参考。

Abstract:

OBJECTIVE To analyse Polygoni Cuspidati Rhizoma et Radix from different areas by HPLC-DAD, and to establish the method for identification and quality control of it. METHODS YMC-C₁₈ column (4.6 mm×250 mm, 5 μm) was used. Analysis was performed on condition of gradient elution solvents of acetonitrile and 0.2% phosphoric acid. The flow rate was 1.0 mL·min^-1, detecting wavelength was 280 nm and column temperature was 25 ℃. Eight components of five batches of Polygoni Cuspidati Rhizoma et Radix were determined, and the fingerprint chromatogram of it was established. RESULTS The method of content determination was desirable precision, reproducibility, and stability. Seventeen common peaks were established from specimens, the similarity of samples was 0.915-0.973. CONCLUSION This method indicates the difference of the chemical component in Polygoni Cuspidati Rhizoma et Radix from various habitats in China, and can be used for quality control.