| 引用本文: | 李樱红,汪瑾,胡磊,熊英,罗金文.HPLC测定红曲黄酮片中总蒽醌的含量[J].中国现代应用药学,2012,29(12):1131-1133、1139. |
| LI Yinghong,WANG Jin,HU Lei,XIONG Ying,LUO Jinwen .Determination of Anthraquinone in Hongquhuangtong Pill by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(12):1131-1133、1139. |
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| 摘要: |
| 目的 建立高效液相色谱法测定红曲黄酮片中总蒽醌的含量。方法 以Waters Sunfire C18(250 mm×4.6 mm，5 μm)为色谱柱，甲醇-乙腈-0.1%磷酸(25∶40∶35)为流动相，流速1.0 mL·min-1，柱温35 ℃，检测波长254 nm。结果 色谱峰分离度良好，大黄素在11.50~115.04 ng、大黄酚在12.24~122.40 ng、大黄素甲醚在4.87~48.71 ng内呈良好线性关系。平均加样回收率大黄素为100.6%，大黄酚为101.4%，大黄素甲醚为97.7%。结论 该方法简单、快速、重复性好，可用于红曲黄酮片中总蒽醌的质量控制。 |
| 关键词: 总蒽醌 决明子 大黄素 大黄酚 大黄素甲醚 |
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| Determination of Anthraquinone in Hongquhuangtong Pill by HPLC |
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LI Yinghong1, WANG Jin2, HU Lei1, XIONG Ying2, LUO Jinwen 1
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1.Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China;2.Zhejiang Hangzhou Xinfu Pharmaceutical Co.,Ltd, Hangzhou 311300, China
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| Abstract: |
| OBJECTIVE To establish a method for determining the content of anthraquinone in Hongquhuangtong pill by HPLC. METHODS Waters sunfire C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was consisted of methanol, acetonitrile and 0.1% phosphoric acid (25∶40∶35). The flow rate was 1.0 mL·min-1. The column temperature was set at 35 ℃. The detector wavelength was 254 nm. RESULTS Emodin, chrysophanol, and physcion showed a good linear relationship with range in 11.50-115.04 ng, 12.24-122.40 ng, and 4.87-48.71 ng, respectively; and the recovery were 100.6%, 101.4%, and 97.7%. CONCLUSION The method is simple, rapid, accurate and reliable. And it can be applied to control the quality of anthraquinone from Hongquhuangtong pill. |
| Key words: anthraquinone Cassiae Semen emodin chrysophanol physcion |
