| 引用本文: | 白雪媛,赵雨,张惠,王思明,李晓华.不同产地人参中水溶性蛋白质含量的差异性研究[J].中国现代应用药学,2012,29(11):980-983. |
| BAI Xueyuan,ZHAO Yu,ZHANG Hui,WANG Siming,LI Xiaohua.Differences Study of Water-soluble Protein Content in Ginseng from Different Origins[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(11):980-983. |
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| 摘要: |
| 目的 针对长白山区15个不同产地人参中水溶性蛋白成分的含量进行比较研究，以鉴别人参质量的地域性差别。方法 采用SDS-PAGE获得人参中水溶性蛋白的电泳胶片，通过凝胶成像软件将胶片转化成浓度-比移值图谱，累计叠加不同产地的谱图，比较分析不同条带的浓度变化情况。结果 不同产地人参中水溶性蛋白的SDS-PAGE电泳谱带条数无明显差别，主要谱带为13条，但因浓度的不同而引起的谱带的指纹信息差异较大。结论 人参水溶性蛋白的含量在不同产地间存在差异，与地域性密切相关。 |
| 关键词: 人参 不同产地 水溶性蛋白 聚丙烯酰胺凝胶电泳 |
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| 基金项目:国家科技重大专项(2011ZX09401-305-02)；国家科技支撑计划(2012BAI29B05)；吉林省医药产业发展专项(YYZX201134) |
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| Differences Study of Water-soluble Protein Content in Ginseng from Different Origins |
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BAI Xueyuan, ZHAO Yu, ZHANG Hui, WANG Siming, LI Xiaohua
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Changchun University of Chinese Medicine, Changchun 130117, China
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| Abstract: |
| OBJECTIVE Study differences in water-soluble protein content from 15 origins in Changbai Mountain area to identify the quality of ginseng from different origins. METHODS The electrophoresis spectras about ginseng water-soluble protein was got with SDS-PAGE. The electrophoresis spectras were transfened through the gel imaging software into concentration Rf spectras. The spectra of ginseng protein from different origins was superimposed and concentration of spectras were compared. RESULTS The amount of main absorption peak was 13, and the number of absorption peaks were similar, but the fingerprint informations about the contents were different with the different origins. CONCLUSION The contents of water-soluble protein in ginseng are different from different origins. |
| Key words: ginseng different regions water-soluble protein SDS-PAGE |
