HPLC-ELSD测定益母草颗粒中盐酸水苏碱的含量

Determination of Stachydrine Hydrochloride in Leonuri Granule by HPLC-ELSD

关键词:  益母草颗粒  盐酸水苏碱  含量测定  高效液相色谱-蒸发光散射检测器法

Key words:  leonuri granule  stachydrine hydrochloride  content determination  HPLC-ELSD

摘要:

目的 建立HPLC-ELSD测定益母草颗粒中盐酸水苏碱的含量。方法 色谱柱为Venusil HILIC丙基酰胺键合硅胶柱(250 mm×4.6 mm，5 μm)；柱温为25 ℃；流动相为乙腈-0.2%冰醋酸溶液(80∶20)；流速为0.8 mL·min^-1；进样量为10 μL；漂移管温度为80℃，氮气流速为1 L·min^-1。结果 盐酸水苏碱在0.646 5~12.93 μg内呈良好的线性关系，r=0.999 4(n=5)；平均加样回收率为98.48%，RSD=1.15%(n=9)。结论 该方法简便、快速、准确、重复性和稳定性好，可作为益母草颗粒的盐酸水苏碱含量测定方法。

Abstract:

OBJECTIVE To establish an HPLC-ELSD method for the determination of stachydrine hydrochloride content in Herba leonuri granule. METHODS The Venusil HILIC column(250 mm×4.6 mm, 5 μm) was adopted. The column temperature was 25 ℃. The mobile phase was acetonitrile-0.2% glacial acetic acid(80∶20). The flow rate was 0.8 mL·min^-1. The injection volume was 10 μL. The drift tube temperature was 80 ℃, and the nebulizer gas flow rate was 1 L·min^-1. RESULTS The linear range of stachydrine hydrochloride was 0.646 5-12.93 μg, r=0.999 4(n=5). The average recovery was 98.48%, RSD=1.15%(n=9). CONCLUSION The HPLC-ELSD method is convenient, rapid and accurate with good reproducibility and stability which is suitable for assay of stachydrine hydrochloride content in leonuri granule.