超高效液相色谱-串联质谱测定保健食品中马兜铃酸A

Determination of Aristolochic Acid A in Health Food by UHPLC-MS/MS

关键词:  保健食品  马兜铃酸A  超高效液相色谱-串联质谱法

Key words:  health food  aristolochic acid A  UHPLC-MS/MS

摘要:

目的 建立测定保健食品中马兜铃酸A的超高效液相色谱-串联质谱法。方法 采用Zorbax Eclipse Plus C₁₈(2.1 mm×100 mm，1.8 μm)；流动相为乙腈-0.1%甲酸(含10 mmol·L^-1甲酸铵)(60∶40)；流速：0.2 mL·min^-1；质谱条件为电喷雾电离源(ESI⁺)，以多重反应监测(MRM)方式进行检测，用于定量分析的反应离子为m/z 359→324。结果 马兜铃酸A在2~200 ng·mL^-1内线性关系良好，方法平均回收率为105.3%，RSD为0.8%，最低检测限为0.2 μg·kg^-1。结论 本法专属性强，灵敏度和准确度高，可用于保健食品中马兜铃酸A的测定。

Abstract:

OBJECTIVE To establish a UHPLC-MS/MS method for the determination of aristolochic acid A in health food. METHODS The separation and analysis was performed on a Zorbax Eclipse Plus C₁₈ column(2.1 mm×100 mm, 1.8 μm). The mobile phase consisted of acetonitrile-0.1% fomic acid containing 10 mmol·L^-1 ammonium formate(60∶40). The flow rate was 0.2 mL·min^-1. Electrospray ionization(ESI) source was applied and operated in the positive mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 359→324 was used to quantify aristolochic acid A. RESULTS The assay linearity of aristolochic acid A was confirmed over the range 2-200 ng·mL^-1. The average recovery of aristolochic acid A was 105.3% and RSD was 0.8%. The detection limit was 0.2 μg·kg^-1. CONCLUSION The method is proved to be specific, sensitive and accurate. Therefore, it can be used to detect aristolochic acid A in health food.