丹参酮对兔急性心梗再灌注损伤的心肌保护

Myocardium Protection of Tanshinone on Ischemia Reperfusion Injury of Rabbits with Acute Myocardial Infarction

关键词:  丹参酮  心肌缺血再灌注损伤  心肌保护

Key words:  tanshinone  ischemia reperfusion injury  myocardium protection

摘要:

目的 探讨丹参酮在心肌梗死再灌注损伤中的干预作用，为临床治疗提供实验依据。方法 将40只，♂，新西兰大白兔按成组设计应用随机列表法分成4组，每组10只：葡萄糖再灌注组、丹参酮再灌注治疗组、丹参酮冠脉结扎前治疗组和假手术组。建立再灌注模型后经耳缘静脉给药。分别于结扎前5 min、结扎后1 h、再灌注后2 h时，取血清检测肌酸激酶(CK)、肌酸激酶的同功酶(CK-MB)、肌钙蛋白Ⅰ(Tn-Ⅰ)。并于再灌注后2 h，以1%的四氮唑红溶液制作心肌切片，以AutoCAD 2002图形设计软件计算坏死心肌范围。结果 ①丹参酮再灌注组与葡萄糖再灌注组、丹参酮结扎前治疗组与葡萄糖再灌注组在再灌注后2 h的CK、CK-MB、Tn-Ⅰ有统计学差异(P<0.05)；丹参酮再灌注组与丹参酮结扎前治疗组在再灌注后2 h的CK、CK-MB无统计学差异(P>0.05)、Tn-Ⅰ有统计学差异(P<0.05)。②丹参酮再灌注组与葡萄糖再灌注组、丹参酮结扎前治疗组与葡萄糖再灌注组心肌梗死范围比较有统计学差异(P<0.05)。丹参酮再灌注组与丹参酮结扎前治疗组无统计学差异(P>0.05)。结论 静脉注射丹参酮可以降低兔急性心肌梗死再灌注后CK、CK-MB、Tn-Ⅰ，并减少兔急性心肌梗死面积。

Abstract:

OBJECTIVE To explore the effect of tanshinone on ischemia reperfusion injury of rabbits after acute myocardial infarction, and to supply the experimental proof for the clinic. METHODS Divide 40 male rabbits into 4 groups, 10 per group: glucose reperfusion group, tanshinone reperfusion group, tanshinone pre-blocking treatment group and sham-operation group. Reperfusion injury model was established and the drug was administrated intravenously. Blood was collected to test the CK, CK-MB, Tn-Ⅰ at the time of 5 min pre-blocking, 1 h post-blocking and 2 h post reperfusion. 1% Triphenyltetrazolium chloride was used to produce myocardial incision. AutoCAD 2002 software was applied to calculate the area of myocardial infarction. RESULTS ①Tanshinone reperfusion group and glucose reperfusion group had significant differences in CK, CK-MB, Tn-Ⅰ after 2 h reperfusion(P<0.05). Tanshinone pre-blocking treatment group and glucose reperfusion group had significant differences in CK, CK-MB, Tn-Ⅰ after 2 h reperfusion(P<0.05). Tanshinone reperfusion group and tanshinone pre-blocking treatment group had no significant differences in CK, CK-MB after 2 h reperfusion(P>0.05), and had significant differences in Tn-Ⅰ after 2 h reperfusion(P<0.05). ②The area of myocardial infarction between tanshinone reperfusion group and glucose reperfusion group, tanshinone pre-blocking treatment group and glucose reperfusion group had significant differences(P<0.05). The area of myocardial infarction between tanshinone reperfusion group and tanshinone pre-blocking treatment group had no significant differences(P>0.05). CONCLUSION Tanshinone can lower CK, CK-MB, Tn-Ⅰ of rabbits with acute myocardial infarction, and can decrease the area of myocardial infarction.