| 引用本文: | 刘东辉,杨丽娥,黄月纯,魏刚.铁皮枫斗HPLC特征图谱研究[J].中国现代应用药学,2013,30(4):390-394. |
| LIU Donghui,YANG Li’e,HUANG Yuechun,WEI Gang.Analysis of HPLC Characteristic Spectrum on Dendrobium Officinale of Fengdous[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(4):390-394. |
|
| 摘要: |
| 目的 建立铁皮枫斗HPLC特征图谱。方法 采用Zorbax SB C18色谱柱；流动相为乙腈-0.2%的甲酸溶液，梯度洗脱；检测波长为270 nm；柱温为35 ℃；流速为1.0 mL·min-1。结果 铁皮枫斗标示出36个特征共有峰，10批样品的相似度为0.898~0.969。结论 本方法准确可靠，重复性好，为铁皮枫斗的质量控制提供了一定的方法依据。 |
| 关键词: 铁皮枫斗 特征图谱 高效液相色谱法 |
| DOI： |
| 分类号: |
| 基金项目:广东省产学研合作项目(2009B090300333)；广东省产学研结合项目(2011B090400319) |
|
| Analysis of HPLC Characteristic Spectrum on Dendrobium Officinale of Fengdous |
|
LIU Donghui1, YANG Li’e1, HUANG Yuechun2, WEI Gang1
|
|
1.Guangzhou University of TCM, Guangzhou 510006, China;2.The First Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510405, China
|
| Abstract: |
| OBJECTIVE To establish the method of characteristic spectrum analysis on Dendrobium officinale of Fengdous by HPLC. METHODS HPLC was used with the Zorbax SB C18 column, the acetonitrile-0.2%methanoic acid solution with gradient elution was employed as a mobile phase, detection wavelength was 270 nm, column temperature was 35 ℃, and flow rate was 1.0 mL·min-1. RESULTS Thirty six common peaks were separated in Dendrobium officinale of Fengdous, the similarities of 10 batches were 0.898-0.969. CONCLUSION The method is accurate and reliable, has a better reproducibility, and provides a reference for the quality control of Dendrobium officinale of Fengdous. |
| Key words: Dendrobium officinale species of Fengdous characteristic spectrum HPLC |
